CAJ | 학술논문

以1年生合作杨扦插苗为试验材料,利用茉莉酸甲酯(MeJA)分别与水杨酸甲酯(MeSA)、顺式己烯醛(cis-hexenal)、反式己烯醛(trarts-hexenal)和苯骈噻唑(benzothiazole)混合的4种挥发物熏蒸合作杨叶片,检测过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)3种防御酶的活性变化.结果表明:经过4种混合挥发物熏蒸后,合作杨叶片中POD,PPO和PAL活性都有明显的升高,并且2种挥发物混合的诱导效果要高于单独一种挥发物,但不仅仅是每一种挥发物诱导效果的相加之和.从诱导酶活增加的效果来看,在这4种混合挥发物中,MeJA+cis-hexenal的诱导能力最高.
이1년생합작양천삽묘위시험재료,이용말리산갑지(MeJA)분별여수양산갑지(MeSA)、순식기희철(cis-hexenal)、반식기희철(trarts-hexenal)화분병새서(benzothiazole)혼합적4충휘발물훈증합작양협편,검측과양화물매(POD)、다분양화매(PPO)급분병안산해안매(PAL)3충방어매적활성변화.결과표명:경과4충혼합휘발물훈증후,합작양협편중POD,PPO화PAL활성도유명현적승고,병차2충휘발물혼합적유도효과요고우단독일충휘발물,단불부부시매일충휘발물유도효과적상가지화.종유도매활증가적효과래간,재저4충혼합휘발물중,MeJA+cis-hexenal적유도능력최고.
The signal transfer between plants was mediated by compound signals composed of different volatiles. In order to explore the action mode of compound signals, one-year-old poplar( Populus simonii x P. pyramidalis ' Opera 8277 ) cuttings were exposed to four mixed volatiles, i.e. methyl jasmonate(MeJA) + methyl salicylate(MeSA) , MeJA + cis-hexenal, MeJA + trans-hexenal, MeJA + benzothiazole, and then the induced activities of three defensive enzymes including peroxidase (POD) , polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL) were measured. The results showed that all of four mixed volatiles exposure resulted in higher activities of POD, PPO and PAL than only single volatile treatment in intact poplar leaves, indicating that mixed volatiles possessed stronger inducing capability. Moreover the synthetic inducing effect of mixed volatiles was more than the sum of individual volatiles. Among these four mixed volatiles, MeJA + cm-hexenal exhibited the highest inducing capability.