时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
封3-封4
,共1页
痛泻要方%抗炎镇痛止泻%PGE_2/cAMP信号通路
痛瀉要方%抗炎鎮痛止瀉%PGE_2/cAMP信號通路
통사요방%항염진통지사%PGE_2/cAMP신호통로
Tongxie Yaofang%Anti-inflammatory%Antipain and anti-diarrhea%PGE_2/cAMP signal path
目的 探讨痛泻要方的抗炎镇痛作用机制.方法 采用二甲苯致小鼠耳肿胀法观察抗炎作用;采用酶联免疫法测定冰醋酸致痛小鼠血清中前列腺素E_2(PGE_2)和环磷酸腺苷(cAMP)的浓度;采用大黄致泻法观察止泻作用.结果 痛泻要方对二甲苯致小鼠耳肿胀、对冰醋酸致痛小鼠均有显著抑制作用;并明显降低冰醋酸致痛小鼠血清中PGE_2和cAMP浓度;能延长小鼠排黑粪的潜伏期,明显减少黑粪排出数量.结论 痛泻要方具有良好抗炎镇痛止泻作用,PGE_2/cAMP信号通路参与了痛泻要方的抗炎镇痛作用.
目的 探討痛瀉要方的抗炎鎮痛作用機製.方法 採用二甲苯緻小鼠耳腫脹法觀察抗炎作用;採用酶聯免疫法測定冰醋痠緻痛小鼠血清中前列腺素E_2(PGE_2)和環燐痠腺苷(cAMP)的濃度;採用大黃緻瀉法觀察止瀉作用.結果 痛瀉要方對二甲苯緻小鼠耳腫脹、對冰醋痠緻痛小鼠均有顯著抑製作用;併明顯降低冰醋痠緻痛小鼠血清中PGE_2和cAMP濃度;能延長小鼠排黑糞的潛伏期,明顯減少黑糞排齣數量.結論 痛瀉要方具有良好抗炎鎮痛止瀉作用,PGE_2/cAMP信號通路參與瞭痛瀉要方的抗炎鎮痛作用.
목적 탐토통사요방적항염진통작용궤제.방법 채용이갑분치소서이종창법관찰항염작용;채용매련면역법측정빙작산치통소서혈청중전렬선소E_2(PGE_2)화배린산선감(cAMP)적농도;채용대황치사법관찰지사작용.결과 통사요방대이갑분치소서이종창、대빙작산치통소서균유현저억제작용;병명현강저빙작산치통소서혈청중PGE_2화cAMP농도;능연장소서배흑분적잠복기,명현감소흑분배출수량.결론 통사요방구유량호항염진통지사작용,PGE_2/cAMP신호통로삼여료통사요방적항염진통작용.
Objective To study anti-inflammatory, analgesic and anti-diarrhea mechanism of Tongxie Yaofang. Methods The dimethylbenzene-caused swelling of mouse ear was used to observe the anti-inflammatory effect. Enzymelinked immunosorbent assay was used to measure the PGE_2 and cAMP levels in serum of aceticacid-induced pain mice. The rhubarb-induced diarrhea was used to observe the anti-diarrhea effect. Results The anti-inflammatory effect on the swelling of mouse ear caused by dimethylbenzene and the anti-diarrhea effect on the rhubarb-induced diarrhea was significant. The PGE_2 and cAMP concentration in blood serum of acetic-acid-induced pain mice was significantly reduced. The delitescence of the mouse's morbus niger excretion was prolonged, and the quantity of the excretion was significantly decreased.Conclusion Tongxie Yaofang has significant anti-inflammatory, antipain and anti-diarrhea effects, and PGE_2/cAMP signal pathway participates in the anti-inflammatory, analgesic effects.
