上海医学
上海醫學
상해의학
SHANGHAI MEDICAL JOURNAL
2010年
1期
104-106,封2
,共4页
王俊珊%刘占举%郑萍%王兴鹏
王俊珊%劉佔舉%鄭萍%王興鵬
왕준산%류점거%정평%왕흥붕
炎症性肠病%溃疡性结肠炎%克罗恩病%胰岛素样生长因子
炎癥性腸病%潰瘍性結腸炎%剋囉恩病%胰島素樣生長因子
염증성장병%궤양성결장염%극라은병%이도소양생장인자
Inflammatory bowel disease Ulcerative Colitis%Crohn's Disease.Insulin-like growth factor-1
目的 通过检测炎症性肠病(IBD)患者血清胰岛素样生长因子(IGF-1)水平以及肠道黏膜组织中IGF-1的表达,探讨IGF-1与IBD活动度和病变部位之间关系.方法 收集2008年1月-10月诊断为IBD的患者75例,其中克罗恩病(CD)30例,溃疡性结肠炎(UC)45例,收集同期行结肠镜检查未见异常且年龄、性别构成与IBD组相近的39例患者作为对照组.根据Harvey和Bradshow标准的疾病活动指数(简化CDAI)及Southerland疾病活动指数(UCAI)对CD和UC患者的疾病程度进行分级.测定所有患者的血清IGF-1水平以及肠道黏膜组织的IGF-1表达.结果 活动期UC和CD患者的血清IGF-1水平分别为(149.37±101.81)、(112.23±45.94)ng/mL;缓解期分别为(159.4±91.63)、(142.89±121.61)ng/mL,均显著低于对照组的(236.00±62.55)ng/mL(P值均<O.05).但活动期与缓解期亚组间的差异均无统计学意义(P值均>0.05).UC组中,全结肠型和部分结肠型患者的血清IGF-1水平分别为(157.56±96.60)、(140.47±101.68)ng/mL,两亚组间的差异无统计学意义(P>0.05).CD组中,结肠累及型和小肠型患者的血清IGF-1水平分别为(97.48±29.35)、(145.90±113.14)ng/mL,两亚组间的差异无统计学意义(P>0.05).IBD组患者的肠道黏膜IGF-1呈高表达,而对照组呈低表达.结论 血清IGF-1对判断IBD的活动度、病变部位的特异性不强.IBD患者的肠壁组织中可见IGF-1蛋白表达显著增强,可能肠间质细胞局部分泌的IGF-1介导了炎症的发生、发展.
目的 通過檢測炎癥性腸病(IBD)患者血清胰島素樣生長因子(IGF-1)水平以及腸道黏膜組織中IGF-1的錶達,探討IGF-1與IBD活動度和病變部位之間關繫.方法 收集2008年1月-10月診斷為IBD的患者75例,其中剋囉恩病(CD)30例,潰瘍性結腸炎(UC)45例,收集同期行結腸鏡檢查未見異常且年齡、性彆構成與IBD組相近的39例患者作為對照組.根據Harvey和Bradshow標準的疾病活動指數(簡化CDAI)及Southerland疾病活動指數(UCAI)對CD和UC患者的疾病程度進行分級.測定所有患者的血清IGF-1水平以及腸道黏膜組織的IGF-1錶達.結果 活動期UC和CD患者的血清IGF-1水平分彆為(149.37±101.81)、(112.23±45.94)ng/mL;緩解期分彆為(159.4±91.63)、(142.89±121.61)ng/mL,均顯著低于對照組的(236.00±62.55)ng/mL(P值均<O.05).但活動期與緩解期亞組間的差異均無統計學意義(P值均>0.05).UC組中,全結腸型和部分結腸型患者的血清IGF-1水平分彆為(157.56±96.60)、(140.47±101.68)ng/mL,兩亞組間的差異無統計學意義(P>0.05).CD組中,結腸纍及型和小腸型患者的血清IGF-1水平分彆為(97.48±29.35)、(145.90±113.14)ng/mL,兩亞組間的差異無統計學意義(P>0.05).IBD組患者的腸道黏膜IGF-1呈高錶達,而對照組呈低錶達.結論 血清IGF-1對判斷IBD的活動度、病變部位的特異性不彊.IBD患者的腸壁組織中可見IGF-1蛋白錶達顯著增彊,可能腸間質細胞跼部分泌的IGF-1介導瞭炎癥的髮生、髮展.
목적 통과검측염증성장병(IBD)환자혈청이도소양생장인자(IGF-1)수평이급장도점막조직중IGF-1적표체,탐토IGF-1여IBD활동도화병변부위지간관계.방법 수집2008년1월-10월진단위IBD적환자75례,기중극라은병(CD)30례,궤양성결장염(UC)45례,수집동기행결장경검사미견이상차년령、성별구성여IBD조상근적39례환자작위대조조.근거Harvey화Bradshow표준적질병활동지수(간화CDAI)급Southerland질병활동지수(UCAI)대CD화UC환자적질병정도진행분급.측정소유환자적혈청IGF-1수평이급장도점막조직적IGF-1표체.결과 활동기UC화CD환자적혈청IGF-1수평분별위(149.37±101.81)、(112.23±45.94)ng/mL;완해기분별위(159.4±91.63)、(142.89±121.61)ng/mL,균현저저우대조조적(236.00±62.55)ng/mL(P치균<O.05).단활동기여완해기아조간적차이균무통계학의의(P치균>0.05).UC조중,전결장형화부분결장형환자적혈청IGF-1수평분별위(157.56±96.60)、(140.47±101.68)ng/mL,량아조간적차이무통계학의의(P>0.05).CD조중,결장루급형화소장형환자적혈청IGF-1수평분별위(97.48±29.35)、(145.90±113.14)ng/mL,량아조간적차이무통계학의의(P>0.05).IBD조환자적장도점막IGF-1정고표체,이대조조정저표체.결론 혈청IGF-1대판단IBD적활동도、병변부위적특이성불강.IBD환자적장벽조직중가견IGF-1단백표체현저증강,가능장간질세포국부분비적IGF-1개도료염증적발생、발전.
Objective To explore the serum level of insulin-like growth factor-1 (IGF-1) and IGF-1 expression in intestinal mucosa of patients with inflammatory bowel disease (IBD),and to investigate the relationship between IGF-1 and the activity and involvement area of IBD. Methods Seventy-five IBD patients,45 with ulcerous colitis (UC) and 30 with Crohn disease (CD),who were treated during Jan. 2008 to Dec. 2008,were included in the present study. The patients were classified according to Bradshow Classification (CDAI) and Southerland Activity Index (UCAI). Meanwhile,39 age- and sex-matched controls were also included in this study. The serum levels of IGF-1 and the expression of IGF-1 in intestinal mucosa were determined. Results The serum levels of IGF-1 in patients with active UC(rep) and CD (rep) were (149.37 ±101.81, ng/mL and (112.23± 45.94, ng/mL,respectively;and those in patients at remission stage of UC(rep) and CD (rep) were (159.4±91.63, ng/mL and (142.89±121.61, ng/mL,respectively; all the 4 numbers were significantly lower that in the controls ([236.00±62.55]ng/mL,P<0.05). But there were no statistical differences between the active and remission stages in the UC and CD groups (P>0.05,. The levels of IGF-1 in full colon involvement and segmental colon involvement patients with UC were not significantly different ([157.56 ± 96.60) ng/mL vs. [140.47 ±101.68) ng/mL,P>0. 05) . The levels of IGF-1 in colonic CD patients and enteric CD patients were not significantly different ([97.48±29.35]ng/mL vs. [145.90± 113. 14]ng/mL,P>0.05). IGF-1 was highly expressed in the mucosa of IBD patients and lowly expressed in the controls (P<0.05). Conclusion Serum IGF-1 level may not specifically demonstrate the activity and involvement of IBD.IGF-1 protein is highly expressed in the mucosa of IBD patients,ndicating that IGF-1 secreted by mesenchymal cells may mediate the development of fibrosis in IBD patients.
