CAJ | 학술논문

采用平板分离法从皖贝母新鲜鳞茎、茎杆和叶中分离出2种内生真菌（A1和B1）,通过菌落形态和ITS序列确定内生菌的种属,通过DNAMAN软件多重序列比对确定与其他真菌的同源关系。结果表明,A1归为被孢霉属,与Mortierella elon-gata的ITS序列同源性高达99.44%;另一种归为青霉属,与Penicillium janthinellum的ITS序列同源性高达98.65%。
채용평판분리법종환패모신선린경、경간화협중분리출2충내생진균（A1화B1）,통과균락형태화ITS서렬학정내생균적충속,통과DNAMAN연건다중서렬비대학정여기타진균적동원관계。결과표명,A1귀위피포매속,여Mortierella elon-gata적ITS서렬동원성고체99.44%;령일충귀위청매속,여Penicillium janthinellum적ITS서렬동원성고체98.65%。
Two kinds of endophytic fungi（named A1 and B1）were separated from fresh Fritilaria anhuiensis bulb,stem and leaf by culture dish.Their taxonomy were determined by morphology analysis and ITS analysis.The relationship with other fungi were determined by DNAMAN analysis.The results indicated that A1 belongs to genus Mortierella and shared 99.44% nucleotide acid identities with Mortierella elongata ITS.B1 belongs to genus Penicillium and shared 98.65% nucleotide acid identities with Penicillium janthinellum ITS.