CAJ | 학술논문

目的：研究两种不同的淋巴细胞分离液对脐带血单核细胞的分离效果及后期对CIK细胞的诱导培养的影响。方法：分别使用国产TBD以及进口GE healthcare牌淋巴细胞分离液分离提取脐带血单核细胞,用悬浮细胞培养法诱导培养脐带血单核细胞形成CIK细胞,再用血球计数板及台盼兰染色法检测细胞密度及活率。结果：国产的TBD牌所分离的单核细胞分层较清楚,并且方便提取,而使用进口GE healthcare的淋巴细胞分离液提取的单核细胞数量较多,但经过后期诱导培养CIK细胞发现两种分离液提取的细胞培养到后期数量逐渐接近。结论：国产TBD牌淋巴细胞分离液在用于脐带血淋巴细胞分离提取时较进口GE healthcare牌经济,并且细胞提取方便,后期培养效果无明显差别。
목적：연구량충불동적림파세포분리액대제대혈단핵세포적분리효과급후기대CIK세포적유도배양적영향。방법：분별사용국산TBD이급진구GE healthcare패림파세포분리액분리제취제대혈단핵세포,용현부세포배양법유도배양제대혈단핵세포형성CIK세포,재용혈구계수판급태반란염색법검측세포밀도급활솔。결과：국산적TBD패소분리적단핵세포분층교청초,병차방편제취,이사용진구GE healthcare적림파세포분리액제취적단핵세포수량교다,단경과후기유도배양CIK세포발현량충분리액제취적세포배양도후기수량축점접근。결론：국산TBD패림파세포분리액재용우제대혈림파세포분리제취시교진구GE healthcare패경제,병차세포제취방편,후기배양효과무명현차별。
Objective：To compare the result of umbilical cord blood mononuclear cells extracted by two different lymphocyte separation liquids,and the later effect on CIK culture.Methods：The improved methods were used to extract mononuclear cells from umbilical cord blood.Then the mononuclear cells were inducted and cultured to CIK cells.Lastly,the blood corpuscle count plate and trypan blue were used to determination the density and live rate of CIK cells.Results：Compared with the GE healthcare,the quantity of mononuclear cells separated by TBD was less,while the stratification was clearer and more convenient to extract.Besides,when the cells cultured to CIK later,their density and live rate were close gradually.Conclusion：The TBD lymphocyte separation fluid was more economical and convenient than GE healthcare when used to separate mononuclear cells from umbilical cord blood;in addition,there were no significant difference on later culture.