中华普通外科学文献（电子版）
中華普通外科學文獻（電子版）
중화보통외과학문헌（전자판）
CHINESE JOURNAL OF GENERAL SURGERY(ELECTRONIC VERSION)
2012年
5期
19-22
,共4页
王天宝%戴强生%尉秀清%林维浩%石汉平%董文广
王天寶%戴彊生%尉秀清%林維浩%石漢平%董文廣
왕천보%대강생%위수청%림유호%석한평%동문엄
CXCR4%RNA干扰%SW480%细胞周期
CXCR4%RNA榦擾%SW480%細胞週期
CXCR4%RNA간우%SW480%세포주기
CXCR4%RNA interference%SW480%Cell cycle
目的 筛选细胞表面趋化因子受体(CXCR4)RNA干扰高效序列,探讨其对SW480细脆生长的影响.方法 采用RT-PCR和Western blot方法选择最有效的CXCR4 RNA干扰序列,流式细胞术分析转染该序列后SW480细胞生长情况.结果 sihCXCR4-3序列可使CXCR4RNA相对含量仅为SW480组的5.4％,CXCR4蛋白相对表达量为18.95％. sihCXCR4-3组细胞数[(0.92±0.06)×105]低于SW480细胞组[(1.38±0.04)×105,P=0.0050]及NCsihCXCR4组[(1.28±0.05)×105,P=0.0256]；相对SW480组和NCsihCXCR4组,sihCXCR4-3组的抑制率分别为33.03％及28.00％.sihCXCR4-3组G1期百分率明显低于SW480细胞组[(59.5±0.57)％vs.(74.11±0.54)％,P=0.0167]；S期百分率高于SW480组[(23.85±0.24)％vs.(14.62±0.46)％,P=0.0305]和NCsihRNA组[(17.63±0.48)％,P=0.0013].结论 sihCXCR4-3序列可高效沉默CXCR4基因表达,抑制SW480细胞生长.
目的 篩選細胞錶麵趨化因子受體(CXCR4)RNA榦擾高效序列,探討其對SW480細脆生長的影響.方法 採用RT-PCR和Western blot方法選擇最有效的CXCR4 RNA榦擾序列,流式細胞術分析轉染該序列後SW480細胞生長情況.結果 sihCXCR4-3序列可使CXCR4RNA相對含量僅為SW480組的5.4％,CXCR4蛋白相對錶達量為18.95％. sihCXCR4-3組細胞數[(0.92±0.06)×105]低于SW480細胞組[(1.38±0.04)×105,P=0.0050]及NCsihCXCR4組[(1.28±0.05)×105,P=0.0256]；相對SW480組和NCsihCXCR4組,sihCXCR4-3組的抑製率分彆為33.03％及28.00％.sihCXCR4-3組G1期百分率明顯低于SW480細胞組[(59.5±0.57)％vs.(74.11±0.54)％,P=0.0167]；S期百分率高于SW480組[(23.85±0.24)％vs.(14.62±0.46)％,P=0.0305]和NCsihRNA組[(17.63±0.48)％,P=0.0013].結論 sihCXCR4-3序列可高效沉默CXCR4基因錶達,抑製SW480細胞生長.
목적 사선세포표면추화인자수체(CXCR4)RNA간우고효서렬,탐토기대SW480세취생장적영향.방법 채용RT-PCR화Western blot방법선택최유효적CXCR4 RNA간우서렬,류식세포술분석전염해서렬후SW480세포생장정황.결과 sihCXCR4-3서렬가사CXCR4RNA상대함량부위SW480조적5.4％,CXCR4단백상대표체량위18.95％. sihCXCR4-3조세포수[(0.92±0.06)×105]저우SW480세포조[(1.38±0.04)×105,P=0.0050]급NCsihCXCR4조[(1.28±0.05)×105,P=0.0256]；상대SW480조화NCsihCXCR4조,sihCXCR4-3조적억제솔분별위33.03％급28.00％.sihCXCR4-3조G1기백분솔명현저우SW480세포조[(59.5±0.57)％vs.(74.11±0.54)％,P=0.0167]；S기백분솔고우SW480조[(23.85±0.24)％vs.(14.62±0.46)％,P=0.0305]화NCsihRNA조[(17.63±0.48)％,P=0.0013].결론 sihCXCR4-3서렬가고효침묵CXCR4기인표체,억제SW480세포생장.
Objective To select an effective CXCR4 RNA interference sequence and investigate its influence to the growth of SW480.Methods RT-PCR and Western blot were employed to find out the ideal RNA interference sequence,flow cytometry was used to analyze the growth and cell cycle of SW480.Results The relative expression of CXCR4 gene and protein in sihCXCR4-3 group was 5.4％ and 18.95％,resprectively.The mean of SW480 cell was lower in sihCXCR4-3 group [(0.92 ± 0.06) × 105]than in SW480 group[(1.38 ± 0.04) × 105,P=0.0050] and NCsihCXCR4 group[(1.28 ± 0.05) × 105,P =0.0256]. Comparing with SW480 and NCsihCXCR4 groups,the inhibion rate of sihCXCR4-3 group was 33.03％ and 28.00％, respectively. G2 stage percentage in sihCXCR4-3 group was obviously lower than in SW480 group[(59.5 ± 0.57)％ vs.(74.11 ± 0.54)％,P =0.0167],while S stage percentage was higher than in SW480 group [(23.85 ± 0.24)％ vs.(14.62 ± 0.46)％,P =0.0305] and in NCsihRNA group[(17.63 ± 0.48)％,P =0.0013].Conclusion sihCXCR4-3 inhibits effectively the expression of CXCR4 gene and cell proliferation of SW480.
