中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
7期
575-577
,共3页
阿尔茨海默病%神经微丝蛋白质类
阿爾茨海默病%神經微絲蛋白質類
아이자해묵병%신경미사단백질류
Alzheimer disease%Neurofilament proteins
目的 探讨阿尔茨海默病(AD)患者尿液中Alzheimer相关的神经丝蛋白(AD7c-NTP)的含量在AD诊断中的价值. 方法 收集就诊于我院门诊AD患者及健康体检者的尿液标本450例,其中AD组共257例,轻度131例、中重度126例,健康对照组193例,应用双抗体夹心酶联免疫吸附法测定尿样标本中AD7c-NTP水平. 结果 轻度、中重度AD组尿样标本中AD7c-NTP含量为(1.94±0.74)μg/L和(3.92±0.86)μg/L,健康对照组为(0.65±0.80)μg/L,轻度和中重度AD组比较,差异均有统计学意义(t=1.727,P＜0.001),三组比较差异有统计学意义(F=-13.520,P＜0.001).轻度AD组尿样标本中AD7c-NTP含量与MMSE呈负相关(r=-0.23,P=0.006),而中重度AD组尿样标本中AD7c-NTP含量与MMSE无明显的相关性(r=0.59,P=0.113).根据ROC曲线,确定1.50 μg/L为尿AD7c-NTP诊断AD的切点,对应的灵敏度和特异度分别为90.6％和91.8％,ROC曲线下面积为0.94,95％可信区间为0.91～0.97. 结论 尿AD7c-NTP含量检测可作为AD辅助诊断的方法之一.
目的 探討阿爾茨海默病(AD)患者尿液中Alzheimer相關的神經絲蛋白(AD7c-NTP)的含量在AD診斷中的價值. 方法 收集就診于我院門診AD患者及健康體檢者的尿液標本450例,其中AD組共257例,輕度131例、中重度126例,健康對照組193例,應用雙抗體夾心酶聯免疫吸附法測定尿樣標本中AD7c-NTP水平. 結果 輕度、中重度AD組尿樣標本中AD7c-NTP含量為(1.94±0.74)μg/L和(3.92±0.86)μg/L,健康對照組為(0.65±0.80)μg/L,輕度和中重度AD組比較,差異均有統計學意義(t=1.727,P＜0.001),三組比較差異有統計學意義(F=-13.520,P＜0.001).輕度AD組尿樣標本中AD7c-NTP含量與MMSE呈負相關(r=-0.23,P=0.006),而中重度AD組尿樣標本中AD7c-NTP含量與MMSE無明顯的相關性(r=0.59,P=0.113).根據ROC麯線,確定1.50 μg/L為尿AD7c-NTP診斷AD的切點,對應的靈敏度和特異度分彆為90.6％和91.8％,ROC麯線下麵積為0.94,95％可信區間為0.91～0.97. 結論 尿AD7c-NTP含量檢測可作為AD輔助診斷的方法之一.
목적 탐토아이자해묵병(AD)환자뇨액중Alzheimer상관적신경사단백(AD7c-NTP)적함량재AD진단중적개치. 방법 수집취진우아원문진AD환자급건강체검자적뇨액표본450례,기중AD조공257례,경도131례、중중도126례,건강대조조193례,응용쌍항체협심매련면역흡부법측정뇨양표본중AD7c-NTP수평. 결과 경도、중중도AD조뇨양표본중AD7c-NTP함량위(1.94±0.74)μg/L화(3.92±0.86)μg/L,건강대조조위(0.65±0.80)μg/L,경도화중중도AD조비교,차이균유통계학의의(t=1.727,P＜0.001),삼조비교차이유통계학의의(F=-13.520,P＜0.001).경도AD조뇨양표본중AD7c-NTP함량여MMSE정부상관(r=-0.23,P=0.006),이중중도AD조뇨양표본중AD7c-NTP함량여MMSE무명현적상관성(r=0.59,P=0.113).근거ROC곡선,학정1.50 μg/L위뇨AD7c-NTP진단AD적절점,대응적령민도화특이도분별위90.6％화91.8％,ROC곡선하면적위0.94,95％가신구간위0.91～0.97. 결론 뇨AD7c-NTP함량검측가작위AD보조진단적방법지일.
Objective To investigate the value of Alzheimer-associated neuronal thread protein (AD7c-NTP) level in urine for diagnosing Alzheimer's disease (AD). Methods The urine samples of 450 subjects were collected from out-department of our hospital.There were 257 people with AD diseases (131 mild cases,126 moderate and severe cases) and 193 healthy control.ELISA was applied to test the level of AD7c-NTP in urine samples. Results The levels of AD7c-NTP were (1.94±0.74)μg/L,(3.92 ± 0.86 ) μg/L and (0.65 ± 0.80) μg/L in mild AD,moderate and severe AD,healthy control groups,respectively.There were differences among three groups(F=-13.520,P＜0.001),and between mild and moderate and severe AD(t =1.727,P＜ 0.001).The level of AD7c-NTP was negatively related with MMSE score in mild AD (r =- 0.23,P =0.006),while no correlation was found between AD7c-NTP and MMSE in moderate and severe AD(r=0.59,P =0.113).Using receiver operating characteristic curve(ROC curve),the optimal cutoff point of AD7cNTP in urine for diagnosis of AD was 1.50 μg/L,with 90.6％ sensitivity and 91.8％ specificity,area under the curve was 0.94(95％ CI:0.91-0.97). Conclusions The level of AD7c-NTP in urine may be one of parameters for diagnosing AD.
