CAJ | 학술논문

目的探讨脑缺血预处理对脑缺血大鼠血管生成素-1(angiop oietin-1,Ang-1)及其受体Tie-2 mRNA表达的影响.方法 99只Wistar大鼠随机分成假手术组(n=9)、非缺血预处理组(nonischemic preconditioning,NIP)(n=45)和缺血预处理组(ischemic preconditioning,IP)(n=45),后两组再随机分为缺血再灌注1d、3d、7d、14 d和21 d等5个亚组(每组n=9).线栓法建立短暂性大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型进行局灶性缺血预处理(缺血10min后恢复灌注).2,3,5-氯化三苯基四氮唑染色法测定脑梗死体积.原位杂交法检测Ang-1/Tie-2 mRNA表达水平.结果 IP组1d、3d和7 d亚组梗死体积显著小于NIP组相应亚组(P均＜0.01),IP组3d和7 d亚组Ang1 mRNA以及1d、3 d和7 d亚组Tie-2 mRNA表达较NIP组显著性上调(P均＜0.05).IP组3 d亚组梗死体移缩小最显著(P＜0.05),7d亚组Ang-1 mRNA表达显著上调,而其受体Tie-2 mRNA表达高峰出现在IP后3d,并持续至7 d.Pearson相关性分析显示,IP组Ang-1/Tie-2 mRNA表达水平与梗死体积呈显著性负相关(P＜0.01).结论 Ang-1和Tie-2 mRNA在缺血预处理后产生脑缺血耐受时间窗内(预处理后1～7 d)表达上调,其中Ang-1可能主要作用于脑缺血耐受的后期阶段.
목적 탐토뇌결혈예처리대뇌결혈대서혈관생성소-1(angiop oietin-1,Ang-1)급기수체Tie-2 mRNA표체적영향.방법 99지Wistar대서수궤분성가수술조(n=9)、비결혈예처리조(nonischemic preconditioning,NIP)(n=45)화결혈예처리조(ischemic preconditioning,IP)(n=45),후량조재수궤분위결혈재관주1d、3d、7d、14 d화21 d등5개아조(매조n=9).선전법건립단잠성대뇌중동맥폐새(middle cerebral artery occlusion,MCAO)모형진행국조성결혈예처리(결혈10min후회복관주).2,3,5-록화삼분기사담서염색법측정뇌경사체적.원위잡교법검측Ang-1/Tie-2 mRNA표체수평.결과 IP조1d、3d화7 d아조경사체적현저소우NIP조상응아조(P균＜0.01),IP조3d화7 d아조Ang1 mRNA이급1d、3 d화7 d아조Tie-2 mRNA표체교NIP조현저성상조(P균＜0.05).IP조3 d아조경사체이축소최현저(P＜0.05),7d아조Ang-1 mRNA표체현저상조,이기수체Tie-2 mRNA표체고봉출현재IP후3d,병지속지7 d.Pearson상관성분석현시,IP조Ang-1/Tie-2 mRNA표체수평여경사체적정현저성부상관(P＜0.01).결론 Ang-1화Tie-2 mRNA재결혈예처리후산생뇌결혈내수시간창내(예처리후1～7 d)표체상조,기중Ang-1가능주요작용우뇌결혈내수적후기계단.
Objective To investigate the effect of cerebral ischemic preconditioning (IP) on the expressions of angiopoietin-1 (Ang-1) and its receptor Tie-2 mRNA in cerebral ischemia in rats.Methods Ninety-nine Wistar rats were randomly assigned to three groups:sham operation (n =9),non-ischemic preconditioning (NIP) (n =45),and IP (n =45).The latter two groups were redivided into 5 subgroups:ischemia-reperfusion 1,3,7,14,and 21 days (n =9 in each group).A model of transient middle cerebral artery occlusion (MCAO) was induced by the intraluminal suture method for focal IP (ischemia for 10 minutes and restoring perfusion).Infarct volume was determined by 2,3,5-triphenyltetrazolium staining.The expression levels of Ang-1/Tie-2 mRNA were detected by in situ hybridization.Results The infarct volumes in the 1 -,3-,and 7-day subgroups of the IP group were significantly smaller than those in the relative subgroups of the NIP group (all P＜ 0.05).The expression of Ang-1 mRNA in the 3- and 7-day subgroups of the IP group and the expression of Tie-2 mRNA in the 1-,3-,and 7-day subgroups of the NIP group were upregulated significantly (all P ＜ 0.05).The infarct volume in the 3-day subgroup of the IP group was reduced most significantly (P ＜ 0.05).The expression of Ang-1 mRNA in the 7-day subgroup was upregulated significantly,and the peak expression of its receptor Tie-2 mRNA appeared at day 3 after IP and continued to day 7.Pearson correlation analysis showed that the expression levels of Ang-1/Tie-2 mRNA were significantly negatively correlated with infarct volume (P ＜0.01).Conclusions The expression of Ang-1/Tie-2 mRNA in the IP group was upregulated within the time window of ischemic tolerance (1 - 7 days after preconditioning),in which Ang-1 may mainly act on the later stage of the cerebral ischemic tolerance.