CAJ | 학술논문

目的探讨不同浓度甘草甜素对肝星状细胞增殖的影响及其调控机制的研究.方法用MTT法检测三组不同浓度甘草甜素对肝星状细胞增殖的抑制率,用免疫细胞化学法检测细胞周期素cyclinE的表达情况.结果 MTT法检测不同药物浓度组:空白对照组、0.8 mg/L甘草甜素组、1.2 mg/L甘草甜素组、1.6 mg/L甘草甜素组细胞增殖抑制率分别为0,2.73%,14.75%,25.96%,差异有统计学意义(P＜0.05);不同浓度甘草甜素组中cyclinE阳性表达细胞数与对照组比较明显减少,且呈浓度依赖性,差异有统计学意义(P＜0.01).结论甘草甜素能够浓度依赖性的抑制大鼠肝星状细胞增殖;甘草甜素可通过降低细胞周期素cyclinE的表达从而抑制大鼠肝星状细胞增殖.
목적 탐토불동농도감초첨소대간성상세포증식적영향급기조공궤제적연구.방법 용MTT법검측삼조불동농도감초첨소대간성상세포증식적억제솔,용면역세포화학법검측세포주기소cyclinE적표체정황.결과 MTT법검측불동약물농도조:공백대조조、0.8 mg/L감초첨소조、1.2 mg/L감초첨소조、1.6 mg/L감초첨소조세포증식억제솔분별위0,2.73%,14.75%,25.96%,차이유통계학의의(P＜0.05);불동농도감초첨소조중cyclinE양성표체세포수여대조조비교명현감소,차정농도의뢰성,차이유통계학의의(P＜0.01).결론 감초첨소능구농도의뢰성적억제대서간성상세포증식;감초첨소가통과강저세포주기소cyclinE적표체종이억제대서간성상세포증식.
Objective To explore the different concentrations of glycyrrhizin on hepatic stellate cell proliferation and its regulation mechanism.Methods Hepatic stellate cell proliferation rate of three different concentrations of glycyrrhizin was detected by MTT assay.The expression of cyclin E was detected by immunocytochemistry.Results MTT assay was used to detect the cell proliferation inhabitation in different concentration groups,from control group 0.8 mg/L glycyrrhizin group to 1.2 mg/L glycyrrhizin group and 1.6 mg/L glycyrrhizin group.The cell proliferation inhibited rates were 0,2.73%,14.75 % and 25.96%.The difference was significant (P＜0.05).The numbers of Cyclin E positive cells in different concentrations of glycyrrhizin group were significantly different compared with control group,and it was concentration dependently decreased (P＜0.01).Conclusions Glycyrrhizin concentration dependently inhibited rat hepatic stellate cells proliferation and it could reduce the expression of cyclin E and inhibit the proliferation of rat hepatic stellate cells.