CAJ | 학술논문

目的探讨结缔组织生长因子(CTGF)在胰腺癌中的表达及其对胰腺癌细胞增殖和转移的影响.方法采用实时定量聚合酶链反应(PCR)和免疫组化方法,分别检测胰腺癌细胞株PANC-1和50例胰腺癌组织中CTGF的表达.采用重组腺病毒介导的CTGF转染PANC-1细胞使CTGF高表达,以RNA干扰方法使CTGF表达缺失,分别通过四甲基偶氮唑蓝(MMT)法、划痕实验、体外侵袭实验观察PANC-1细胞增殖和转移能力的变化.结果实时定量PCR和免疫组化检测均显示,CTGF在PANC-1细胞及胰腺癌组织中高表达.CTGF转染后,CTGF转染组PANC-1细胞比空载体对照组增殖能力和修补划痕能力明显增强,200倍显微镜视野下的穿膜细胞数为34个,比空载体对照组(11个)明显增多.干扰CTGF后,siCTGF转染组细胞比空载体对照组增殖能力和修补划痕能力明显降低,200倍显微镜视野下的穿膜细胞数为6个,比空载体对照组(15个)明显减少.结论 CTGF在胰腺癌中高表达,其表达的高低对胰腺癌细胞的增殖和转移有显著影响.
목적 탐토결체조직생장인자(CTGF)재이선암중적표체급기대이선암세포증식화전이적영향.방법 채용실시정량취합매련반응(PCR)화면역조화방법,분별검측이선암세포주PANC-1화50례이선암조직중CTGF적표체.채용중조선병독개도적CTGF전염PANC-1세포사CTGF고표체,이RNA간우방법사CTGF표체결실,분별통과사갑기우담서람(MMT)법、화흔실험、체외침습실험관찰PANC-1세포증식화전이능력적변화.결과 실시정량PCR화면역조화검측균현시,CTGF재PANC-1세포급이선암조직중고표체.CTGF전염후,CTGF전염조PANC-1세포비공재체대조조증식능력화수보화흔능력명현증강,200배현미경시야하적천막세포수위34개,비공재체대조조(11개)명현증다.간우CTGF후,siCTGF전염조세포비공재체대조조증식능력화수보화흔능력명현강저,200배현미경시야하적천막세포수위6개,비공재체대조조(15개)명현감소.결론 CTGF재이선암중고표체,기표체적고저대이선암세포적증식화전이유현저영향.
Objective To explore the expression of connective tissue growth factor(CTGF)in pancreatic cancer and its influence on the proliferation and migration of cancer cells.Methods The expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma(50 cases)tissues by immunohistochemistry.The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay,scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.Results CTGF was overexpressed in both pancreatic cancer cells and tissues.Overxpression of CTGF leads to increased proliferation and migration of PANC-1 cells.The CTGF-transfected PANC-1 cells showed apparent stronger proliferation ability and scratch-repair ability than that of empty vector controls.The results of Boyden chamber test showed that there were 34 cells/field(200 × magnificantion)of the CTGF-transfected overexpressing cells,much more than the 11 cells/field of the empty vector control cells; and 6 cells/micmscopic field of the Ad5-siCTGF-transfected silenced cells,much less than the 15 cells/field of the control cells.Contusions CTGF is overexpressed in both pancreatic cancer cells in vitro and in vivo,indicating that it may play an important role in the cell proliferation and migration in pancreatic cancer.