CAJ | 학술논문

随着蛋白质组学技术的兴起,疾病生物标志物的研究发展迅速,但筛选出的海量候选标志物却极少进入临床应用.近几年,基于MRM-MS技术的方法和策略凭借其低成本、耗时短、高灵敏度、高准确性等优势引起了国内外检验医学界的广泛关注.基于MRM-MS的技术,特别是SISCAPA技术,可能在不远的将来将逐步发展成为蛋白质生物标志物研究的快车道.本文分析了当前生物标志物研究领域的瓶颈,系统阐述了MRM-MS技术的原理和特点,以及近年来该技术在蛋白质生物标志物研究领域的主要应用现状,并展望其发展前景.
수착단백질조학기술적흥기,질병생물표지물적연구발전신속,단사선출적해량후선표지물각겁소진입림상응용.근궤년,기우MRM-MS기술적방법화책략빙차기저성본、모시단、고령민도、고준학성등우세인기료국내외검험의학계적엄범관주.기우MRM-MS적기술,특별시SISCAPA기술,가능재불원적장래장축보발전성위단백질생물표지물연구적쾌차도.본문분석료당전생물표지물연구영역적병경,계통천술료MRM-MS기술적원리화특점,이급근년래해기술재단백질생물표지물연구영역적주요응용현상,병전망기발전전경.
With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application.Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy.MRM-MS based approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future.In this review, the "bottleneck" existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.