CAJ | 학술논문

目的测定病理性瘢痕组织中CD105标记的微血管密度(microvesiel density,MVD)和survivin mRNA的表达,探讨病理性瘢痕组织中癌基因在血管形成中的作用.方法采用多相寡核苷酸探针原位杂交技术和免疫组织化学染色技术(SP法),分别检测survivin mRNA、CD105蛋白在40例病理性瘢痕、20例非病理性瘢痕和20例正常皮肤组织中的表达,并计数CD105标记的MVD.结果 CD105-MVD、survivin mRNA在病理性瘢痕较非病理性瘢痕和正常皮肤的表达差异皆具有统计学意义(P<0.05).病理性瘢痕组中survivin mRNA表达与CD105-MVD表达有相关性(P<0.05).结论病理性瘢痕组织中,凋亡抑制基因survivin表达上调可能参与了其血管形成过程.
목적 측정병이성반흔조직중CD105표기적미혈관밀도(microvesiel density,MVD)화survivin mRNA적표체,탐토병이성반흔조직중암기인재혈관형성중적작용.방법 채용다상과핵감산탐침원위잡교기술화면역조직화학염색기술(SP법),분별검측survivin mRNA、CD105단백재40례병이성반흔、20례비병이성반흔화20례정상피부조직중적표체,병계수CD105표기적MVD.결과 CD105-MVD、survivin mRNA재병이성반흔교비병이성반흔화정상피부적표체차이개구유통계학의의(P<0.05).병이성반흔조중survivin mRNA표체여CD105-MVD표체유상관성(P<0.05).결론 병이성반흔조직중,조망억제기인survivin표체상조가능삼여료기혈관형성과정.
Objective To study the mierovessel density (MVD) marked by CD105 and expression of survivin gene in pathologic scar, and to investigate the function of oncogenes in tumorigenic process and abnormal scarring. Methods In situ hybridization and immunohistoehemical methods (SP technique) were used to detect survivin mRNA, CD105-MVD in 40 pathologic scar cases, 20 normotrophic scar cases, and 20 normal skin cases, respectively. The relationship between the expression of survivin mRNA and CD105-MVD in 40 pathologic scar cases were analyzed. Results Compared with normotrophic scar and normal skin cases, the expression of survivin mRNA, CD105-MVD were significant difference in pathologic scar cases (P<0.05). In pathologic scar tissue, the expression of survivin had significant correlation with MVD marked by CD105 (P<0.05). Conclusion survivin is up-regulated in pathologic scar, which may contribute to the vascular formation.