中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
18期
17-18
,共2页
肠易激综合征%5-羟色胺%免疫组织化学
腸易激綜閤徵%5-羥色胺%免疫組織化學
장역격종합정%5-간색알%면역조직화학
Irritable bowel syndrome%5-hydroxytryptamine%Immunohistochemistry
目的 检测肠易激综合征(IBS)患者肠黏膜5-羟色胺(5-HT)表达的差异,探讨外周5-HT在IBS发病中的作用.方法 选择IBS腹泻型(D-IBS)21例、便秘型(C-IBS)22例患者及正常对照组19例,利用免疫组化方法检测各组回肠、近端结肠、远端结肠黏膜5-HT阳性细胞,计数阳性细胞数量并进行分析.结果 D-IBS组回肠、近端结肠、远端结肠5-HT阳性细胞数显著多于对照组(P<0.05),并且回肠黏膜阳性细胞数也多于C-IBS组(P<0.05),C-IBS组回肠、近端结肠、远端结肠阳性细胞显著多于对照组(P<0.05).结论 肠道5-HT的增加与IBS发病相关,IBS不同亚型发病机制可能与5-HT不同受体在中枢神经系统和消化道表达分布有一定关系.
目的 檢測腸易激綜閤徵(IBS)患者腸黏膜5-羥色胺(5-HT)錶達的差異,探討外週5-HT在IBS髮病中的作用.方法 選擇IBS腹瀉型(D-IBS)21例、便祕型(C-IBS)22例患者及正常對照組19例,利用免疫組化方法檢測各組迴腸、近耑結腸、遠耑結腸黏膜5-HT暘性細胞,計數暘性細胞數量併進行分析.結果 D-IBS組迴腸、近耑結腸、遠耑結腸5-HT暘性細胞數顯著多于對照組(P<0.05),併且迴腸黏膜暘性細胞數也多于C-IBS組(P<0.05),C-IBS組迴腸、近耑結腸、遠耑結腸暘性細胞顯著多于對照組(P<0.05).結論 腸道5-HT的增加與IBS髮病相關,IBS不同亞型髮病機製可能與5-HT不同受體在中樞神經繫統和消化道錶達分佈有一定關繫.
목적 검측장역격종합정(IBS)환자장점막5-간색알(5-HT)표체적차이,탐토외주5-HT재IBS발병중적작용.방법 선택IBS복사형(D-IBS)21례、편비형(C-IBS)22례환자급정상대조조19례,이용면역조화방법검측각조회장、근단결장、원단결장점막5-HT양성세포,계수양성세포수량병진행분석.결과 D-IBS조회장、근단결장、원단결장5-HT양성세포수현저다우대조조(P<0.05),병차회장점막양성세포수야다우C-IBS조(P<0.05),C-IBS조회장、근단결장、원단결장양성세포현저다우대조조(P<0.05).결론 장도5-HT적증가여IBS발병상관,IBS불동아형발병궤제가능여5-HT불동수체재중추신경계통화소화도표체분포유일정관계.
Objective To investigate the role of 5-hydroxytryptamine (5-HT)in patients with irritable bowel syndrome (IBS). Methods By immunohistochemieal assay, the 5-HT levels were detected in intestinal mucosa in control group, D-IBS ( diarrhea IBS) group and C-IBS (constipation IBS) group. Results The number of 5-HT positive cell at ileum, proximate colon, distal colon were significantly more than that of control group, and the number of 5-HT positive cell at ileum was even more than C-IBS group. The number of 5-HT positive cell at ileum, proximate colon, distal colon in C-IBS were higher that of control. Conclusions The enchanced 5-HT in intestine is related to the IBS pathological mechanism, while the occurrence of different IBS subgroups is probably related to the difference expression or distribution of receptors of 5-HT in center nerve systerm and gut.
