CAJ | 학술논문

为了证实人胎肝细胞在体外与丙型肝炎病毒（HCV）阳性血清共培养24 h是否能感染HCV病毒而作定位研究。采用透射电镜、免疫电镜的手段对于HCV阳性血清共培养的人胎肝细胞中病毒样颗粒进行定位研究，以测定细胞内是否有HCV。结果发现在透视电镜下人胎肝细胞胞浆中有很多病毒样颗粒，直径约45 nm。通过免疫电镜进一步证实在人胎肝细胞中这些病毒样颗粒含有HCV基因表达产物NS3及NS5。此外，人胎肝细胞在体外与HCV阳性血清共培养24 h并经多次洗涤后的培养上清加入到新鲜分离的正常人胎肝细胞培养基中继续培养，可以得出上述相同的结果。这些结果表明人胎肝细胞在体外培养情况下可以感染HCV，并且感染了HCV的人胎肝细胞可以产生具有传染性的HCV颗粒分泌到培养液中。
위료증실인태간세포재체외여병형간염병독（HCV）양성혈청공배양24 h시부능감염HCV병독이작정위연구。채용투사전경、면역전경적수단대우HCV양성혈청공배양적인태간세포중병독양과립진행정위연구，이측정세포내시부유HCV。결과발현재투시전경하인태간세포포장중유흔다병독양과립，직경약45 nm。통과면역전경진일보증실재인태간세포중저사병독양과립함유HCV기인표체산물NS3급NS5。차외，인태간세포재체외여HCV양성혈청공배양24 h병경다차세조후적배양상청가입도신선분리적정상인태간세포배양기중계속배양，가이득출상술상동적결과。저사결과표명인태간세포재체외배양정황하가이감염HCV，병차감염료HCV적인태간세포가이산생구유전염성적HCV과립분비도배양액중。
Orientation researches were carried out to confirm whether human fetal hepatocytes (HFH) might be infected with hepatitis C virus (HCV) after co-culturing with HCV positive serum in vitro for 24 h. Under transmission and immune electron microscopy, the virus-like particles in the HFH co-cultured with HCV positive serum were located to determinate whether there were HCV in the cells. It was found that there were lots of virus-like particles with about 45 nm in diameter in cytoplasm of the HFH under transmission electron microscopy. It was further confirmed that these virus-like particles in HFH contained HCV gene expression products NS3 and NS5 under immune electron microscopy. Moreover, the culture supernatant of HFH co-cultured with HCV positive serum for 24 h in vitro with many times of washing was added into the culture medium of the HFH separated freshly and continued to culture, the same results mentioned above were obtained. These results indicated that HFH may be infected with HCV in the circumstances of culture in vitro and the HFH infected with HCV can produce contagious HCV particles to secrete into the culture supernatant.