中国化学工程学报（英文版）
中國化學工程學報（英文版）
중국화학공정학보（영문판）
CHINESE JOURNAL OF CHEMICAL ENGINEERING
2006年
4期
505-510
,共6页
雷引林%金志华%童微星%姚善泾%朱自强
雷引林%金誌華%童微星%姚善涇%硃自彊
뢰인림%금지화%동미성%요선경%주자강
expanded bed adsorption%2-chloropropionic acid%dehalogenase%cellulose%ion exchange
The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U·ml-1 adsorbent, and two active peaks were eluted respectively at 0.15mol· L-1 and 0.3mol· L-1 (NH4)2SO4. The result indicated that the overall enzyme yield was 68%,with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.
