CAJ | 학술논문

建立了植物源性食品中多菌灵、噻菌灵和苯菌灵的高效液相色谱多残留简捷并可靠的检测方法.样品经乙腈提取,MCX固相萃取柱净化后,采用HPLC法测定,以甲醇-0.1%甲酸水溶液为流动相,1.0 mL/min等度洗脱,在0.1～20μg/mL范围内,多菌灵和噻菌灵的峰面积与其浓度呈线性相关,R≥0.9998,检出限均为25μg/kg,添加回收率91.0%～103.5%,变异系数0.44%～8.96%.
건립료식물원성식품중다균령、새균령화분균령적고효액상색보다잔류간첩병가고적검측방법.양품경을정제취,MCX고상췌취주정화후,채용HPLC법측정,이갑순-0.1%갑산수용액위류동상,1.0 mL/min등도세탈,재0.1～20μg/mL범위내,다균령화새균령적봉면적여기농도정선성상관,R≥0.9998,검출한균위25μg/kg,첨가회수솔91.0%～103.5%,변이계수0.44%～8.96%.
A simple,quick and reliable method was established to determine carbendazim,thiabendazole and benomyl residues in food derived from plants by HPLC.The benomyl residue was calculated as its main degradation product-carbendazim.Extracted with acetonitrile and then decontaminated bV MCX solid phase extraction cartridge,the sample was detected bv HPLC.Methanol:0.1%formic acid(30:70,v/v)solution was used as a mobile phase and isocratic elution was conducted at 1.0 mL/min.Within 0.1～20μg/mL there was a good linear correlation(R≥0.9998)between the peak areas and cOncentrations of carbendazion and thiabendazole.Bv this method the detection limits for carbendazim and thiabendazole were 25 ug/kg,the mean recovery rate was between 91.0%～103.5%,and the coefficient of variation 0.44%～8.96%.