中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2009年
11期
996-998,1002
,共4页
韦颖华%吴继周%吴健林%李国坚%陈茂伟%玉艳红%吴霜%龚星光%孟艳丽%虞艳琦
韋穎華%吳繼週%吳健林%李國堅%陳茂偉%玉豔紅%吳霜%龔星光%孟豔麗%虞豔琦
위영화%오계주%오건림%리국견%진무위%옥염홍%오상%공성광%맹염려%우염기
基因重组乙肝疫苗%HLA-DRB1*07,13%免疫应答
基因重組乙肝疫苗%HLA-DRB1*07,13%免疫應答
기인중조을간역묘%HLA-DRB1*07,13%면역응답
Hepatitis B vaccine%HLA-DRB1*07,13%Immune response
目的:探讨机体HLA-DRB1*07,13等位基因对基因重组乙肝疫苗免疫效果的影响.方法:选取完成基因重组乙肝疫苗全程接种(10 μg/次,0、1、6月)的广西籍汉族健康大学生896名,于末次接种疫苗后的第6个月采血检测血清抗-HBs水平,对无或低应答者再次接种基因重组乙肝疫苗20 μg,4周后筛选出无或低应答者99名及初次检测中或强应答者136名作为研究对象,应用PCR-SSP技术对研究对象外周血HLA-DRB1*07,13等位基因进行检测.结果:HLA-DRB107在无或低应答组中的表达频率为16.16%,显著高于中或强应答组的表达频率(4.41%)(P＜0.05);HLA-DRB1*13在无或低应答组和中或强应答组的表达频率分别为1.01%和3.68%,两组比较无统计学差异(P＞0.05).结论:基因重组乙肝疫苗无或低应答与HLA-DRB1*07基因密切相关;而HLA-DRB1*13对乙肝疫苗的免疫应答无明显影响.
目的:探討機體HLA-DRB1*07,13等位基因對基因重組乙肝疫苗免疫效果的影響.方法:選取完成基因重組乙肝疫苗全程接種(10 μg/次,0、1、6月)的廣西籍漢族健康大學生896名,于末次接種疫苗後的第6箇月採血檢測血清抗-HBs水平,對無或低應答者再次接種基因重組乙肝疫苗20 μg,4週後篩選齣無或低應答者99名及初次檢測中或彊應答者136名作為研究對象,應用PCR-SSP技術對研究對象外週血HLA-DRB1*07,13等位基因進行檢測.結果:HLA-DRB107在無或低應答組中的錶達頻率為16.16%,顯著高于中或彊應答組的錶達頻率(4.41%)(P＜0.05);HLA-DRB1*13在無或低應答組和中或彊應答組的錶達頻率分彆為1.01%和3.68%,兩組比較無統計學差異(P＞0.05).結論:基因重組乙肝疫苗無或低應答與HLA-DRB1*07基因密切相關;而HLA-DRB1*13對乙肝疫苗的免疫應答無明顯影響.
목적:탐토궤체HLA-DRB1*07,13등위기인대기인중조을간역묘면역효과적영향.방법:선취완성기인중조을간역묘전정접충(10 μg/차,0、1、6월)적엄서적한족건강대학생896명,우말차접충역묘후적제6개월채혈검측혈청항-HBs수평,대무혹저응답자재차접충기인중조을간역묘20 μg,4주후사선출무혹저응답자99명급초차검측중혹강응답자136명작위연구대상,응용PCR-SSP기술대연구대상외주혈HLA-DRB1*07,13등위기인진행검측.결과:HLA-DRB107재무혹저응답조중적표체빈솔위16.16%,현저고우중혹강응답조적표체빈솔(4.41%)(P＜0.05);HLA-DRB1*13재무혹저응답조화중혹강응답조적표체빈솔분별위1.01%화3.68%,량조비교무통계학차이(P＞0.05).결론:기인중조을간역묘무혹저응답여HLA-DRB1*07기인밀절상관;이HLA-DRB1*13대을간역묘적면역응답무명현영향.
Objective:To investigate the effect of HLA-DRB1*07,13 allelic genes on the immune response to hepatitis B vaccination.Methods:896 healthy college students of Han nationality from Guangxi province,who had received standard courses of vaccination with recombinant hepatitis B virus vaccine,were tested the level of anti-HBs with ELISA method at the sixth month after their last vaccination.The non- or hypo-responders were selected to receive another 20 μg doses of recombinant vaccine and were examined anti-HBs once more four weeks later.99 non- or hypo-responders and 136 medium or hyper-responders were selected for the study subjects.HLA-DRB1*07,13 allelic genes of the subjects were detected by polymerase chain reaction using sequence specific primer (PCR-SSP) method.Results:The frequencies of HLA-DRB1*07 gene in the non- or hypo-responders group were significantly higher than that in the medium or hyper-responder group (16.16% vs 4.41%,P＜0.05). No significant difference of HLA-DRB1*13 gene between the two groups (1.01% vs 3.68%,P=0.389).Conclusion:There is a close relationship between the expression of HLA-DRB1*07 gene and the non- or hypo-response to HB vaccine;No relationship between HLA-DRB1*13 gene expression and the response to HB vaccine is found.
