CAJ | 학술논문

目的体外分离培养、鉴定兔骨髓基质干细胞(BMSCs)并探讨其分化潜能.方法应用密度梯度离心联合贴壁筛选的方法,体外分离培养兔骨髓来源的骨髓基质干细胞,培养过程中倒置显微镜下观察其生物学特性,HE染色和CD34、CD44免疫组织化学染色行细胞学鉴定;应用体外成骨与成脂诱导分化检测其生物学活性.结果体外实验成功分离培养兔BMSCs,HE染色显示细胞均一性好;CD44强阳性表达,CD34阴性表达.诱导成骨结果显示BMSCs具有较强的成骨活性,ALP染色阳性;成脂诱导结果显示BMSCs具有较强的成脂活性,油红"O"脂肪染色阳性.结论 BMSCs体外具有潜在的多向分化能力.
목적 체외분리배양、감정토골수기질간세포(BMSCs)병탐토기분화잠능.방법 응용밀도제도리심연합첩벽사선적방법,체외분리배양토골수래원적골수기질간세포,배양과정중도치현미경하관찰기생물학특성,HE염색화CD34、CD44면역조직화학염색행세포학감정;응용체외성골여성지유도분화검측기생물학활성.결과 체외실험성공분리배양토BMSCs,HE염색현시세포균일성호;CD44강양성표체,CD34음성표체.유도성골결과현시BMSCs구유교강적성골활성,ALP염색양성;성지유도결과현시BMSCs구유교강적성지활성,유홍"O"지방염색양성.결론 BMSCs체외구유잠재적다향분화능력.
Objective To observe the effect of the in vitro isolation, culture and identification of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) and explore the differentiation potentials of BMSCs. Methods BMSCs were isolated and purified by density gradient centrifugation combined with attachment culture method. The biological characteristics of BMSCs were observed under an inverted microscope. The BMSCs were identified with HE staining and CD34/CD44 immunohistochemical staining. The biological activity of BMSCs was detected by osteogenic induction and steatogenic induction. Results BMSCs were isolated successfully in vitro and had good homogenicity. Immunohistochemical staining of CD34/CD44 showed strong positive expression of CD44 and negative expression of CD34. Fortis osteogenic ability and positive staining of ALP could be detected by osteogenic induction, and fortis steatogenic ability and positive staining of axungia could be detected by steatogenic induction. Conclusion BMSCs have the capability of multi-directional differentiation in vitro.