中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2009年
12期
894-898
,共5页
王柏丁%宁允叶%冯玉麟%文富强
王柏丁%寧允葉%馮玉麟%文富彊
왕백정%저윤협%풍옥린%문부강
activin A%A549%MTT分析%流式细胞术%活化素Ⅱ型受体
activin A%A549%MTT分析%流式細胞術%活化素Ⅱ型受體
activin A%A549%MTT분석%류식세포술%활화소Ⅱ형수체
activin A%A549%MTT assay%flow cytometry%type Ⅱ receptors of activin
背景与目的:活化素(activins)是转化生长因子TGF-β超家族成员.有研究表明,活化素可以诱导多种肿瘤细胞的凋亡.本研究旨在探讨重组人activin AM人肺腺痛细胞系A549增殖及凋亡的影响.方法:体外培养A549细胞,以不同浓度activin A处理A549细胞不同时间后,用MTT法检测其生长抑制情况;流式细胞仪及Annexin V-FITC试剂盒检测activin A对A549细胞凋亡的影响;Western blot检测活化素Ⅱ型受体(ActR Ⅱ和ActR ⅡB)的表达情况.结果:aetivin A能抑制A549细胞增殖,且呈剂量和时间依赖性.流式细胞仪检测结果显示,activin A能促进A549细胞凋亡.Western blot结果显示,随着activin A浓度的增加,活化素Ⅱ型受体的表达量呈浓度依赖性增加.结论:activin A能在体外抑制A549细胞的增殖并诱导其凋亡.推测是通过诱导活化素Ⅱ型受体的表达,激活其下游一系列信号转导通路,从而发挥其生物学功能.
揹景與目的:活化素(activins)是轉化生長因子TGF-β超傢族成員.有研究錶明,活化素可以誘導多種腫瘤細胞的凋亡.本研究旨在探討重組人activin AM人肺腺痛細胞繫A549增殖及凋亡的影響.方法:體外培養A549細胞,以不同濃度activin A處理A549細胞不同時間後,用MTT法檢測其生長抑製情況;流式細胞儀及Annexin V-FITC試劑盒檢測activin A對A549細胞凋亡的影響;Western blot檢測活化素Ⅱ型受體(ActR Ⅱ和ActR ⅡB)的錶達情況.結果:aetivin A能抑製A549細胞增殖,且呈劑量和時間依賴性.流式細胞儀檢測結果顯示,activin A能促進A549細胞凋亡.Western blot結果顯示,隨著activin A濃度的增加,活化素Ⅱ型受體的錶達量呈濃度依賴性增加.結論:activin A能在體外抑製A549細胞的增殖併誘導其凋亡.推測是通過誘導活化素Ⅱ型受體的錶達,激活其下遊一繫列信號轉導通路,從而髮揮其生物學功能.
배경여목적:활화소(activins)시전화생장인자TGF-β초가족성원.유연구표명,활화소가이유도다충종류세포적조망.본연구지재탐토중조인activin AM인폐선통세포계A549증식급조망적영향.방법:체외배양A549세포,이불동농도activin A처리A549세포불동시간후,용MTT법검측기생장억제정황;류식세포의급Annexin V-FITC시제합검측activin A대A549세포조망적영향;Western blot검측활화소Ⅱ형수체(ActR Ⅱ화ActR ⅡB)적표체정황.결과:aetivin A능억제A549세포증식,차정제량화시간의뢰성.류식세포의검측결과현시,activin A능촉진A549세포조망.Western blot결과현시,수착activin A농도적증가,활화소Ⅱ형수체적표체량정농도의뢰성증가.결론:activin A능재체외억제A549세포적증식병유도기조망.추측시통과유도활화소Ⅱ형수체적표체,격활기하유일계렬신호전도통로,종이발휘기생물학공능.
Background and purpose: It was reported that activins, members of transforming growth factor-β superfamily, could induce several tumor cells into apoptosis. This study was designed to observe the effects of recombination human activin A on proliferation and apoptosis ofA549 cells. Methods: A549 cells were cultivated by routine method, then treated with different concentrations of recombination human activin A. Inhibitory effect of activin A on A549 cell proliferation was detected by MTT assay, and apoptosis of A549 cells was determined by flow cytometry. The expression of type Ⅱ receptors of activin (ActR Ⅱ and ActR Ⅱ B) was detected by Western blot. Results: Recombination human activin A inhibited the proliferation ofA549 cells in time-dependent and dose-dependent manners. Increases of apoptosis in A549 cells and expression of ActR I] and ActR ⅡB occurred dose-dependently after treated with acfivin A. Conclusion: Recombination human activin A inhibited cell proliferation and induced apoptosis in A549 cells, at least in part, by inducing expression of type Ⅱ receptors of activin and activation of its downstream signaling.