CAJ | 학술논문

目的　为探讨改进大鼠肝脏低温保存方法对鼠肝的影响,将切取后的鼠肝灌入一定量的自制保存液(HYD液)后，结扎进出肝脏的各血管。方法　应用大鼠离体肝灌注模型，对传统保存法(对照组)和改进保存法(实验组:20%组、30%组和40%组)肝脏微循环指标(门静脉灌注压、流出液内皮素-1、台盼蓝分布时间及组织学改变)、流出液有关酶指标水平及分泌胆汁量进行了观察。结果　20%组、30%组及40%组肝脏微循环指标及分泌胆汁量明显优于对照组(P<0.05),30%组肝脏有关酶指标水平明显低于对照组(P<0.05),其中30%组保存肝脏效果最佳。结论　改进法保存鼠肝的各项指标明显优于传统法,故提示本法行之有效，值得临床工作借鉴。
목적　위탐토개진대서간장저온보존방법대서간적영향,장절취후적서간관입일정량적자제보존액(HYD액)후，결찰진출간장적각혈관。 방법　응용대서리체간관주모형，대전통보존법(대조조)화개진보존법(실험조:20%조、30%조화40%조)간장미순배지표(문정맥관주압、류출액내피소-1、태반람분포시간급조직학개변)、류출액유관매지표수평급분비담즙량진행료관찰。 결과　20%조、30%조급40%조간장미순배지표급분비담즙량명현우우대조조(P<0.05),30%조간장유관매지표수평명현저우대조조(P<0.05),기중30%조보존간장효과최가。 결론　개진법보존서간적각항지표명현우우전통법,고제시본법행지유효，치득림상공작차감。
Objective　To investigate the cold preservation effect on rat livers of a modified storage method with self-made HYD solution. Methods　The vascular bed of rat livers was expanded with an additional 20 to 40?ml self-made HYD solution/100?g liver. After resection of the liver, the extra HYD solution (expressed as % liver weight) was entrapped via portal infusion by tying off the supra- and infra-hepatic inferior vena cava. Forty rats were randomly divided into four groups including control group with conventional storage method, and 20%, 30% and 40% groups according to the amount of extra HYD solution. We compared the preservation effect of the modified storage method with that of the conventional storage method using an isolated perfused rat liver model. Results　Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, endothelin-1 in the effluent, trypan blue distribution time and histology were significantly superior in the modified method groups compared to those in the control group (P<0.05). The contents of dihydroxybenzoic acid (DHBA) in the modified method groups were significantly lower than those in the control group (P<0.05). Liver enzymes activities in the 30% group were markedly lower than those in the control group (P<0.05). The preservation effect on rat liver in the 30% group was the best among the modified method groups. Conclusion　The modified cold storage method is effective and may have potential for clinical application in liver preservation.