CAJ | 학술논문

目的了解T淋巴细胞异常在骨髓增生异常综合征(MDS)克隆造血中的作用.方法对76例MDS患者的染色体核型、T淋巴细胞亚群及激活状态进行分析.结果正常核型36例,异常核型40例,异常发生率52.6%.40例异常核型中,三体8(+8)24例,占异常核型的60.0%.与健康对照组比较,MDS患者CD+3 CD-19、CD+3 CD-4 CD+8以及CD+3 HLA-DR+细胞百分率显著升高,CD-3(CD16 CD56)+细胞的百分率明显降低.将MDS患者进行核型分组,异常核型组CD+3(CD16 CD56)+细胞的百分率显著高于正常对照组.将+8核型从MDS异常核型中独立出来进行分析,CD+3 CD+4 CD-8细胞的百分率明显低于正常核型以及其他异常组,CD4/CD8的比值明显低于健康对照组.结论 MDS存在T淋巴细胞异常,异常核型MDS可能恶性克隆增殖更为优势,预后更差.+8核型MDS存在更为严重的免疫监视功能下降,导致恶性克隆过度增殖与残存造血过度受抑.
목적 료해T림파세포이상재골수증생이상종합정(MDS)극륭조혈중적작용.방법 대76례MDS환자적염색체핵형、T림파세포아군급격활상태진행분석.결과 정상핵형36례,이상핵형40례,이상발생솔52.6%.40례이상핵형중,삼체8(+8)24례,점이상핵형적60.0%.여건강대조조비교,MDS환자CD+3 CD-19、CD+3 CD-4 CD+8이급CD+3 HLA-DR+세포백분솔현저승고,CD-3(CD16 CD56)+세포적백분솔명현강저.장MDS환자진행핵형분조,이상핵형조CD+3(CD16 CD56)+세포적백분솔현저고우정상대조조.장+8핵형종MDS이상핵형중독립출래진행분석,CD+3 CD+4 CD-8세포적백분솔명현저우정상핵형이급기타이상조,CD4/CD8적비치명현저우건강대조조.결론 MDS존재T림파세포이상,이상핵형MDS가능악성극륭증식경위우세,예후경차.+8핵형MDS존재경위엄중적면역감시공능하강,도치악성극륭과도증식여잔존조혈과도수억.
Objective To investigate the effect of dysfunction of T lymphocytes on clonal haematogenesis in patients with myelodysplastic syndrome (MDS). Methods The cytogentics, the subsets of lymphocytes and their activation in 76 patients with MDS were analyzed. Results There were 36 patients with normal karyotype and 40 patients with abnormal karyotype. The incidence of abnormal karyotype were 52.6 %. There were 24 cases (60.0 %) with trisomy 8 (+8) in 40 cases of abnormal karyotype. The expression rates of CD+3 CD-19 cells, CD+3 CD-4 CD+8 cells and CD+3 HLA-DR+ cells in MDS were significantly increased, and CD-3 (CD16CD56)+ cells were significantly lower than that in control group. The expression rates of CD+3 (CD16CD56)+ cells in MDS with abnormal karyotype were significantly higher than that in control group. The expression rates of CD+3 CD+4 CD-8 cells in +8 MDS were significantly lower than that in MDS patients with normal karyotype and with other abnormal karyotype. The ratio of CD4/CD8 in +8 MDS were significantly lower than that in control group. Conclusion The abnormalities of T cell subsets and functions in patients with MDS were observed and the proliferation of malignant clone was prevalent which indicated a poor prognosis in MDS with abnormal karyotype. Dysfunction of immunosurveillance was more aggravated in +8 MDS, which led to excess proliferation of malignant clone and over inhibition of remaining haematogenesis.