中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
12期
1067-1069
,共3页
色素上皮衍生因子%血管内皮生长因子%氧化应激%糖尿病肾病%肾小球系膜细胞
色素上皮衍生因子%血管內皮生長因子%氧化應激%糖尿病腎病%腎小毬繫膜細胞
색소상피연생인자%혈관내피생장인자%양화응격%당뇨병신병%신소구계막세포
Pigment epithelium derived factor%Vascular endothelial growth factor%Oxidative stress%Diabetic nephropathy%Human mesangial cells
观察不同浓度高糖培养下及人重组色素上皮衍生因子(PEDF)干预后人肾小球系膜细胞活性氧簇水平、血管内皮生长因子(VEGF)mRNA和蛋白的改变.结果 显示活性氧簇水平、VEGF mRNA和蛋白的表达随糖浓度的升高而增加;PEDF干预后,高糖环境下活性氧簇水平及VEGF的表达明显下降,且呈浓度依赖性.提示PEDF可能通过改善血管通透性和抑制氧化应激延缓糖尿病肾病的进展.
觀察不同濃度高糖培養下及人重組色素上皮衍生因子(PEDF)榦預後人腎小毬繫膜細胞活性氧簇水平、血管內皮生長因子(VEGF)mRNA和蛋白的改變.結果 顯示活性氧簇水平、VEGF mRNA和蛋白的錶達隨糖濃度的升高而增加;PEDF榦預後,高糖環境下活性氧簇水平及VEGF的錶達明顯下降,且呈濃度依賴性.提示PEDF可能通過改善血管通透性和抑製氧化應激延緩糖尿病腎病的進展.
관찰불동농도고당배양하급인중조색소상피연생인자(PEDF)간예후인신소구계막세포활성양족수평、혈관내피생장인자(VEGF)mRNA화단백적개변.결과 현시활성양족수평、VEGF mRNA화단백적표체수당농도적승고이증가;PEDF간예후,고당배경하활성양족수평급VEGF적표체명현하강,차정농도의뢰성.제시PEDF가능통과개선혈관통투성화억제양화응격연완당뇨병신병적진전.
To investigate the expression of vascular endothelial growth factor (VEGF)and the levels of reactive oxygen species (ROS)in human mesangial cells cultured in high glucose and being intervened by pigment epithelium-derived factor (PEDF). The results showed that with increased glucose concentration, the expressions of VEGF and ROS generation also gradually increased and PEDF significantly downregulated VEGF expression and ROS generation in a dose-dependent manner. PEDF may delay the diabetic nephropathy progress by improving vascular permeability and inhibiting oxidative stress.
