CAJ | 학술논문

目的建立大鼠颈上交感神经节细胞的急性方分离法并鉴定其电生理学特性.方法 SD大鼠,雌雄不拘,5～12d龄,断头后,急性分离大鼠的颈上交感神经节,经酶解消化法获得颈上交感神经节细胞.采用全细胞膜片钳技术,于钳制电位-60 mV下,加入100μmol/L乙酰胆碱,记录乙酰胆碱受体电流.采用碳纤维电极技术,于钳制电位760 mV下,加入65 mmol/LKCl,检测儿茶酚胺类物质分泌活动.结果分离出表面光滑、遮光性较好的颈上交感神经节细胞,并记录到乙酰胆碱受体电流和儿茶酚胺类物质的分泌.结论急性分离大鼠颈上交感神经节细胞的方法可获得适于膜片钳实验的细胞.
목적 건립대서경상교감신경절세포적급성방분리법병감정기전생이학특성.방법 SD대서,자웅불구,5～12d령,단두후,급성분리대서적경상교감신경절,경매해소화법획득경상교감신경절세포.채용전세포막편겸기술,우겸제전위-60 mV하,가입100μmol/L을선담감,기록을선담감수체전류.채용탄섬유전겁기술,우겸제전위760 mV하,가입65 mmol/LKCl,검측인다분알류물질분비활동.결과 분리출표면광활、차광성교호적경상교감신경절세포,병기록도을선담감수체전류화인다분알류물질적분비.결론 급성분리대서경상교감신경절세포적방법가획득괄우막편겸실험적세포.
Objective To establish a method for acute isolation of rat superior cervical ganglion (SCG) cells and identify the electrophysiological properties.Methods Sprague-Dawley rats of both sexes,aged 5-12 days,were decapitated.The SCGs were removed quickly,and the single SCG cell was enzymatically isolated from the SCGs.When the holding potential was - 60 mV,100 μmol/L acetylcholine was applied and the nicotinic acetylcholine receptor currents were recorded by whole-cell patch-clamp technique.When the holding potential was 760 mV,65 mmol/L KCl was applied and quantal release of catecholamines was detected by using carbon fiber electrodes.Results SCG cells with normal electrophysiological properties were isolated.Typical nicotinic acetylcholine receptor currents and quantal release of catecholamines were recorded successfully.Conclusion The cells suitable for patch-clamp experiments can be obtained by using the method for acute isolation of rat SCG cells.