CAJ | 학술논문

目的检测急性脑梗死患者CD19+-cD25+、CD19+-CD25-B 淋巴细胞、免疫球蛋白和补体C3的含量并探讨其临床意义.方法根据病史及头颅CT或MRI明确疾病诊断.抽取69例急性脑梗死、115例脑出血患者、41例正常对照者静脉血各4 mL,采用流式细胞仪检测CD19+-CD25+、CD19+-CD25-B淋巴细胞百分比,采用散射比浊法检测免疫球蛋白和补体C3含量,并结合不同的病程、不同影像学评分和不同的神经功能评分进行分析比较.结果脑梗死和脑出血急性期CD19+-CD25+、CD19+-CD25-B淋巴细胞、免疫球蛋白和补体C3的差异无统计学意义(P均＞0.05).脑梗死急性期CD19+-CD25+B淋巴细胞百分比、IgG、补体C3含量均较恢复期及对照组显著增高(P均＜0.05).脑梗死恢复期各项体液免疫指标与对照组之间差异无统计学意义(P均＞0.05).不同影像学评分患者之间CD19+-CD25+、CD19+-CD25-B淋巴细胞百分比差异有统计学意义(P均＜0.05).脑梗死急性期神经功能评分与各体液免疫指标间无相关(P均＞0.05).结论脑梗死与脑出血存在着同样的体液免疫功能改变.这种改变可能与应激、病变部位及病变范围有关.脑梗死病灶越大,体液免疫改变越明显;随着应激的消逝,体液免疫功能逐渐恢复.
목적 검측급성뇌경사환자CD19+-cD25+、CD19+-CD25-B 림파세포、면역구단백화보체C3적함량병탐토기림상의의.방법 근거병사급두로CT혹MRI명학질병진단.추취69례급성뇌경사、115례뇌출혈환자、41례정상대조자정맥혈각4 mL,채용류식세포의검측CD19+-CD25+、CD19+-CD25-B림파세포백분비,채용산사비탁법검측면역구단백화보체C3함량,병결합불동적병정、불동영상학평분화불동적신경공능평분진행분석비교.결과 뇌경사화뇌출혈급성기CD19+-CD25+、CD19+-CD25-B림파세포、면역구단백화보체C3적차이무통계학의의(P균＞0.05).뇌경사급성기CD19+-CD25+B림파세포백분비、IgG、보체C3함량균교회복기급대조조현저증고(P균＜0.05).뇌경사회복기각항체액면역지표여대조조지간차이무통계학의의(P균＞0.05).불동영상학평분환자지간CD19+-CD25+、CD19+-CD25-B림파세포백분비차이유통계학의의(P균＜0.05).뇌경사급성기신경공능평분여각체액면역지표간무상관(P균＞0.05).결론 뇌경사여뇌출혈존재착동양적체액면역공능개변.저충개변가능여응격、병변부위급병변범위유관.뇌경사병조월대,체액면역개변월명현;수착응격적소서,체액면역공능축점회복.
Objective To detect the ratio of CD19+-CD25+ and CD19+-CD25-B lymphocytes and content of IgA,IgG,IgM and complement C3 in patients with acute cerebral infarction and study their clinical significance. Methods Disease were diagnosed according to the history and cranlal computer tomography or magnetic resonance imagine.Venous blood of 69 cases with acute cerebral infarction and 115 cases with cerebral hemorrhage, 41 cases in normal control group was extrdcted. The ratio of CDl9+-CD25+and CD19+-CD25-B lymphocytes was determined by flow cytometry and content of IgA,IgG,IgM and C3 was measured with scattering turbidimetry.Changes in humoral immunological function were compared among patients with different courses of disease, imaging scores and neurological function scores. Results Differences in CD19+-CD25+and CD19+-CD25-B lymphocytes, IgA, IgG,IgM and C3 were not significant at the acute stage between cerebral infarction and cerebral hemorrhage (P＞0.05,for all).The ratio of CD19+-CD25+B lymphocytes and content of IgG and C3 at the acute stage of cerebral infarction were all higher than that at the recovery stage and in the control group (P＜0.05, for all). There was no statistical signmcance in humoral immunological indices between that at the recovery stage of cerebral infarction and in the control group (P＞0.05, for all). The ratio of CD19+-CD25+ and CD19+-CD25-B lymphocytes was significantly different among patients with different imaging scores (P＜0.05,for all).Neurological function scores at the acute stage of cerebrdl inflarction were not correlated with humoral immunological indices(P＞0.05,for all). Conclusions Same changes occur to humoral immunological function in patients with cerebral infarction and cerebral hemomlage, which might be related with stress,and location and scope of lesions.The larger the lesion of cerebral infarction is,the more obvious changes of humoral immunological function become; with the disappearing of stress,humoral immunological function gradually recovers.