CAJ | 학술논문

目的探讨体外培养神经干细胞是否表达趋化因子受体CX3CR1.方法采用无血清方法分离、培养新生大鼠海马神经干细胞,细胞免疫荧光方法检测神经干细胞标志巢蛋白(nestin)表达及干细胞多向分化为神经元及胶质细胞的能力,后通过细胞免疫荧光及RT-PCR方法检测神经干细胞表达趋化因子受体CX3CR1的情况.结果体外培养新生大鼠海马神经干细胞呈nestin阳性.可分化为神经丝蛋白200(NF200)阳性的神经元、胶质纤维酸性蛋白(GFAP)阳性的星形胶质细胞及2',3'-环核苷酸-3'-磷酸二酯酶(CNP)阳性的少突胶质细胞,细胞免疫荧光及RT-PCR证实神经干细胞表达趋化因子受体CX3CR1.结论新生大鼠海马神经干细胞表达趋化因子受体CX3CR1.为进一步研究其体内外迁移提供理论依据.
목적 탐토체외배양신경간세포시부표체추화인자수체CX3CR1.방법 채용무혈청방법분리、배양신생대서해마신경간세포,세포면역형광방법검측신경간세포표지소단백(nestin)표체급간세포다향분화위신경원급효질세포적능력,후통과세포면역형광급RT-PCR방법검측신경간세포표체추화인자수체CX3CR1적정황.결과 체외배양신생대서해마신경간세포정nestin양성.가분화위신경사단백200(NF200)양성적신경원、효질섬유산성단백(GFAP)양성적성형효질세포급2',3'-배핵감산-3'-린산이지매(CNP)양성적소돌효질세포,세포면역형광급RT-PCR증실신경간세포표체추화인자수체CX3CR1.결론 신생대서해마신경간세포표체추화인자수체CX3CR1.위진일보연구기체내외천이제공이론의거.
Objective To explore whether neural stern cells in vitro express the chemokine receptor CX3CR1. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in a serum-flee medium. Using immunofluorescence, the neural stem cells and their differentiation into neural and glial cell types were identified; then, the immunofluorescence and RT-PCR were employed to detect whether the neural stem cells in vitro expressed chemokine receptor CX3CR1. Results The floating neural spheres, isolated from neonatal rat hippocampi, were nestin positive and could differentiate into cells to express characteristic antigens of neurons, astrocytes and oligodendrocytes, Immunofluorescence and RT-PCR showed that neural stem cells expressed chemokine receptor CX3CR1. Conclusion Neural stem cells in vitro can express chemokine receptor CX3CR1, which offers the theoretical evidence for further study of its migration.