CAJ | 학술논문

目的探讨脑脊液中白细胞介素-8(IL-8)、基质金属蛋白酶-9(MMP-9)、干扰素-γ(INF-γ)含量的检测对结核性脑膜炎及病毒性脑膜炎的临床诊断价值. 方法选取解放军总医院、解放军第三0九医院自2010年8月至2011年11月住院的患者,其中结核性脑膜炎组20例,病毒性脑膜炎组15例,非感染性神经系统疾病组20例.用ELISA法检测3组患者脑脊液IL-8、MMP-9、INF-γ含量,并进行比较分析. 结果结核性脑膜炎组患者脑脊液中IL-8、MMP-9、INF-γ的含量高于病毒性脑膜炎组和非感染性神经系统疾病组差异有统计学意义(P＜0.05).病毒性脑膜炎组患者脑脊液中IL-8、MMP-9含量高于非感染性神经系统组(P＜0.05).病毒性脑膜炎组患者脑脊液中INF-γ含量与非感染性神经系统疾病组比较差异无统计学意义(P＞0.05). 结论脑脊液中IL-8、MMP-9、INF-γ含量的检测对结核性脑膜炎具有一定的辅助诊断意义.IL-8、MMP-9在病毒性脑膜炎的发病和进展中亦起到一定作用.临床上若在患者脑脊液中检测到高水平的INF-γ,较之IL-8、MMP-9对于结核性脑膜炎更具诊断价值.
목적 탐토뇌척액중백세포개소-8(IL-8)、기질금속단백매-9(MMP-9)、간우소-γ(INF-γ)함량적검측대결핵성뇌막염급병독성뇌막염적림상진단개치. 방법 선취해방군총의원、해방군제삼0구의원자2010년8월지2011년11월주원적환자,기중결핵성뇌막염조20례,병독성뇌막염조15례,비감염성신경계통질병조20례.용ELISA법검측3조환자뇌척액IL-8、MMP-9、INF-γ함량,병진행비교분석. 결과 결핵성뇌막염조환자뇌척액중IL-8、MMP-9、INF-γ적함량고우병독성뇌막염조화비감염성신경계통질병조차이유통계학의의(P＜0.05).병독성뇌막염조환자뇌척액중IL-8、MMP-9함량고우비감염성신경계통조(P＜0.05).병독성뇌막염조환자뇌척액중INF-γ함량여비감염성신경계통질병조비교차이무통계학의의(P＞0.05). 결론 뇌척액중IL-8、MMP-9、INF-γ함량적검측대결핵성뇌막염구유일정적보조진단의의.IL-8、MMP-9재병독성뇌막염적발병화진전중역기도일정작용.림상상약재환자뇌척액중검측도고수평적INF-γ,교지IL-8、MMP-9대우결핵성뇌막염경구진단개치.
Objective To investigate the diagnostic values of interleukin-8 (IL-8), matrix metalloproteinase-9 (MMP-9) and interferon gamma (INF-γ) levels in patients with tuberculous meningitis and viral meningitis by detecting the contents of these biomarkers in the cerebrospinal fluid (CSF). Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-8,MMP-9 and INF-γ in the CSF of patients with tuberculous meningitis (n=20),viral meningitis (n=15) and noninfectious neurologic diseases (n=20) who admitted to our hospital from August 2010 to November 2011. Results The IL-8,MMP-9 and INF-γlevels in the samples from the tuberculous meningitis patients were significantly higher than those from either viral meningitis or noninfectious neurologic diseases (P＜0.05).The contents of IL-8 and MMP-9 in patients with viral meningitis were significantly higher than those in patients with noninfectious neurologic diseases (P＜0.05),while that of INF-γ showed no significant difference between the 2 groups (P＞0.05). Conclusion Detection of IL-8,MMP-9 and INF-γ in CSF is useful in the diagnosis of tuberculous meningitis; IL-8 and MMP-9 participate in the progress of viral meningitis; the detective value of high concentration of INF-γ in CSF is more significant in diagnosis of tuberculous meningitis than that of IL-8 and MMP-9.