CAJ | 학술논문

目的利用原子力显微镜(AFM)观察经非离子表面活性剂Nonidet P-40(NP-40)不同浓度系列处理的A型流感病毒表面形态变化,观察不同表面活性剂-病毒表面相互作用情况,以提供一种较为温和的病毒表面裂解条件,为利用AFM进一步研究病毒下层结构提供基础.方法用不同浓度的非离子犁表面活性剂NP-40对完整的A型流感病毒进行处理,以轻敲模式经AFM成像,获得病毒球状体和丝状体的高度图、振幅图以及相位图,并观察和比较不同浓度非离子表面活性剂对病毒表面形态和结构的影响.结果 NP-40各浓度对病度表面破坏程度不一,病毒随NP-40浓度增高而逐渐降解,并出现部分剥离病毒表面.暴露下层衣壳,更清晰地展示包膜下层表面突起的表面形态学结果.结论通过表面活性剂优化处理病毒颗粒,实现了利用AFM观测流感病毒包膜下形态结构的设想.
목적 이용원자력현미경(AFM)관찰경비리자표면활성제Nonidet P-40(NP-40)불동농도계렬처리적A형류감병독표면형태변화,관찰불동표면활성제-병독표면상호작용정황,이제공일충교위온화적병독표면렬해조건,위이용AFM진일보연구병독하층결구제공기출.방법 용불동농도적비리자리표면활성제NP-40대완정적A형류감병독진행처리,이경고모식경AFM성상,획득병독구상체화사상체적고도도、진폭도이급상위도,병관찰화비교불동농도비리자표면활성제대병독표면형태화결구적영향.결과 NP-40각농도대병도표면파배정도불일,병독수NP-40농도증고이축점강해,병출현부분박리병독표면.폭로하층의각,경청석지전시포막하층표면돌기적표면형태학결과.결론 통과표면활성제우화처리병독과립,실현료이용AFM관측류감병독포막하형태결구적설상.
Objective Through observing the morphology and topography of the prepared influenza viruses (HIN1) treated with the different Nonidet P-40 solutions using atomic force microscopy (AFM),to explore the application of AFM on the research of the internal character of viral morphology and structural virology. Methods The virus samples were treated with serial diluted Nonidet P-40 solutions from 0.05% to 0.20% and then investigated by AFM with the tapping mode in air at room temperature to obtain the morphology and topography changes including height data,amplitude data and phase data for both spherical and filamentous influenza virus A. Results The serial AFM images show that the erosion degree of the virions is proportional with the improvement of NP-40 concentration,and partly denuded virion image appeared at 0.05 % NP-40 treatment,which was revealed clearly on both amplitude images and phase images. Conclusion This work demonstrated for the first time that the internal topography of influenza virion could be revealed by AFM via suitable nonionic surfactants chemical dissection.