CAJ | 학술논문

目的探讨主要组织相容性复合物Ⅰ类链相关基因(MIC)在溃疡性结肠炎(UC)发病中所起的作用.方法采用荧光定量实时-聚合酶链反应(RT-PCR)方法检测34例UC患者和17例正常人肠黏膜组织中MICA和MICB及其受体NKG2D mRNA表达水平的差异.采用激光共聚焦显微镜观察肠黏膜组织中MICA分子的表达定位.结果 UC组MICA、MICB及NKG2DmRNA水平的表达量(分别为3.5408±2.6658、8.9879±3.2893和2.4395±0.8147)均显著高于正常对照组(分别为1.0477±0.7201、4.6293±1.2616和1.1624±0.3954,P值分别=0.0053、0.0039和0.0078).免疫荧光共聚焦显微镜观察显示,MICA分子在肠上皮细胞胞膜中表达.结论 UC患者肠黏膜组织中MICA、MICB及其受体NKG2D的mRNA表达水平均增高,提示MIC基.因在UC局部起重要作用.
목적 탐토주요조직상용성복합물Ⅰ류련상관기인(MIC)재궤양성결장염(UC)발병중소기적작용.방법 채용형광정량실시-취합매련반응(RT-PCR)방법검측34례UC환자화17례정상인장점막조직중MICA화MICB급기수체NKG2D mRNA표체수평적차이.채용격광공취초현미경관찰장점막조직중MICA분자적표체정위.결과 UC조MICA、MICB급NKG2DmRNA수평적표체량(분별위3.5408±2.6658、8.9879±3.2893화2.4395±0.8147)균현저고우정상대조조(분별위1.0477±0.7201、4.6293±1.2616화1.1624±0.3954,P치분별=0.0053、0.0039화0.0078).면역형광공취초현미경관찰현시,MICA분자재장상피세포포막중표체.결론 UC환자장점막조직중MICA、MICB급기수체NKG2D적mRNA표체수평균증고,제시MIC기.인재UC국부기중요작용.
Objective To investigate the function of major histocompatibility complex (MHC)class Ⅰ-related gene (MIC) in ulcerative colitis (UC) pathogenesis. Methods The difference of MICA, M ICB and their ligand NKG2D genes expression in colonic mucosa tissue of 34 UC patients and 12 healthy people were determined by fluorescent real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and the expression location of M ICA in colonic mucosa tissue was obtained by laser scanning confocal microscope. Results The mRNA level of MICA, MICB and NKG2D expression in UC groups (3. 5408±2. 6658, 8. 9879±3. 2893 and 2. 4395±0. 8147 accordingly) was significantly higher than that in the healthy controls ( 1. 0477 ± 0. 7201, 4. 6293 ± 1. 2616 and 1. 1624±0. 3954 accordingly) (P = 0.0053, 0.0039 and 0. 0078 accordingly). It suggested that MICA was expressed in colonic epithelia cell membranes by laser scanning confocal microscopy.Conclusion The mRNA level of MICA, M ICB, and their ligand, NKG2D expression were all up regulated in the colonic mucosa of UC patients, which indicated MIC gene might perform important local function in UC.