中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2009年
1期
49-52
,共4页
洪帆%庄福连%王彪%林经安
洪帆%莊福連%王彪%林經安
홍범%장복련%왕표%림경안
血管瘤%促血管生成素%Tie-2受体%细胞增殖相关蛋白Ki-67
血管瘤%促血管生成素%Tie-2受體%細胞增殖相關蛋白Ki-67
혈관류%촉혈관생성소%Tie-2수체%세포증식상관단백Ki-67
Hemangiomas%Angiopoietin%Tie-2 reeeptor%Cell proliferation associated pro-tein Ki-67
目的 研究血管瘤组织中促血管生成素-1(Ang-1)、促血管生成素-2(Ang-2)及Tie-2受体的mRNA表达水平,探讨其在血管瘤血管生成中的作用与意义.方法 用反转录-聚合酶链式反应(RT-PCR)的方法 分别检测30例血管瘤组织(增生期血管瘤17例,消退期血管瘤13例)以及10例小儿正常皮肤组织中Ang-1 mRNA、Ang-2 mRNA及Tie-2 mRNA的表达水平.用免疫组织化学方法 检测血管瘤组织Ki-67蛋白的表达.结果 增生期血管瘤组织中Ang-2 mRNA及Tie-2 mRNA表达水平高于消退期血管瘤组织(P<0.01).血管瘤组织中Ang-1 mRNA表达为阴性.结论 Ang-Tie-2体系表达失衡在血管瘤的发生发展过程中可能起重要的作用.
目的 研究血管瘤組織中促血管生成素-1(Ang-1)、促血管生成素-2(Ang-2)及Tie-2受體的mRNA錶達水平,探討其在血管瘤血管生成中的作用與意義.方法 用反轉錄-聚閤酶鏈式反應(RT-PCR)的方法 分彆檢測30例血管瘤組織(增生期血管瘤17例,消退期血管瘤13例)以及10例小兒正常皮膚組織中Ang-1 mRNA、Ang-2 mRNA及Tie-2 mRNA的錶達水平.用免疫組織化學方法 檢測血管瘤組織Ki-67蛋白的錶達.結果 增生期血管瘤組織中Ang-2 mRNA及Tie-2 mRNA錶達水平高于消退期血管瘤組織(P<0.01).血管瘤組織中Ang-1 mRNA錶達為陰性.結論 Ang-Tie-2體繫錶達失衡在血管瘤的髮生髮展過程中可能起重要的作用.
목적 연구혈관류조직중촉혈관생성소-1(Ang-1)、촉혈관생성소-2(Ang-2)급Tie-2수체적mRNA표체수평,탐토기재혈관류혈관생성중적작용여의의.방법 용반전록-취합매련식반응(RT-PCR)적방법 분별검측30례혈관류조직(증생기혈관류17례,소퇴기혈관류13례)이급10례소인정상피부조직중Ang-1 mRNA、Ang-2 mRNA급Tie-2 mRNA적표체수평.용면역조직화학방법 검측혈관류조직Ki-67단백적표체.결과 증생기혈관류조직중Ang-2 mRNA급Tie-2 mRNA표체수평고우소퇴기혈관류조직(P<0.01).혈관류조직중Ang-1 mRNA표체위음성.결론 Ang-Tie-2체계표체실형재혈관류적발생발전과정중가능기중요적작용.
Objective To detect the expression level of Ang-1, Ang -2 and Tie-2 receptor mRNAs in the hemangioma tissuses and to explore the effects of Ang-1, Ang-2, and Tie-2 receptor on the angio-genesis in the hemangioma. Methods By use of semiquantinative reverse transcription polymerase chain reaction, we detected the expression level of Ang-1 mRNA, Ang-2 mRNA and Tie-2 mRNA in 30 cases of hemangioma (17 specimens of proliferating hemangioma, and 13 involuting hemangioma )together with 10 cases of normal skin as control, and the expression level of Ki-67 protein was also detected by an im-munohistochemica method. Results The expression of Ang-2 mRNA and Tie-2 mRNA in the prolifera-ting hemangiomas was higher than that in involuting hemangiomas (P<0.01) . The expression of Ang-1 mRNA in hemangiomas was negative. Conclusion The unbalance of Ang-Tie-2 system is probably impor-tant for the pathogenesis and development in hemangiomas.
