中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2011年
4期
284-289
,共6页
组织工程%成纤维细胞%伤口愈合%皮肤%胚胎
組織工程%成纖維細胞%傷口愈閤%皮膚%胚胎
조직공정%성섬유세포%상구유합%피부%배태
Tissue engineering%Fibroblasts%Wound healing%Skin%Embryo
目的 利用鼠胚胎成纤维细胞(mouse embryonic fibrobalsts,MEFs)三维培养修复大鼠全层皮肤缺损,并初步探讨其机制.方法 培养MEFs和普通成纤维细胞(fibroblasts,FBs),复合鼠尾胶原形成三维构建,ELASA法测定其分泌功能.制作大鼠全层皮肤缺损,按移植物不同分为3组:MEFs组(实验组)、FBs组(对照组1)和空白胶原组(对照组2).观察愈合时间并计算愈合面积比率,定期取创面组织行CD31、波形蛋白免疫组化检测,并以Hoechst33342荧光标记MEFs,示踪其转归.结果 与FBs组相比,MEFs组的IL-6分泌更加旺盛,而TGF-β1分泌量少(P＜0.05).实验组创面愈合快(P＜0.05),微血管密度高(P＜0.05),创面中FBs排列有序,移植的MEFs随时间减少.结论 MEFs三维构建可加速创面愈合,减轻瘢痕形成,其机制可能与胚胎细胞对创面愈合的诱导有关.
目的 利用鼠胚胎成纖維細胞(mouse embryonic fibrobalsts,MEFs)三維培養脩複大鼠全層皮膚缺損,併初步探討其機製.方法 培養MEFs和普通成纖維細胞(fibroblasts,FBs),複閤鼠尾膠原形成三維構建,ELASA法測定其分泌功能.製作大鼠全層皮膚缺損,按移植物不同分為3組:MEFs組(實驗組)、FBs組(對照組1)和空白膠原組(對照組2).觀察愈閤時間併計算愈閤麵積比率,定期取創麵組織行CD31、波形蛋白免疫組化檢測,併以Hoechst33342熒光標記MEFs,示蹤其轉歸.結果 與FBs組相比,MEFs組的IL-6分泌更加旺盛,而TGF-β1分泌量少(P＜0.05).實驗組創麵愈閤快(P＜0.05),微血管密度高(P＜0.05),創麵中FBs排列有序,移植的MEFs隨時間減少.結論 MEFs三維構建可加速創麵愈閤,減輕瘢痕形成,其機製可能與胚胎細胞對創麵愈閤的誘導有關.
목적 이용서배태성섬유세포(mouse embryonic fibrobalsts,MEFs)삼유배양수복대서전층피부결손,병초보탐토기궤제.방법 배양MEFs화보통성섬유세포(fibroblasts,FBs),복합서미효원형성삼유구건,ELASA법측정기분비공능.제작대서전층피부결손,안이식물불동분위3조:MEFs조(실험조)、FBs조(대조조1)화공백효원조(대조조2).관찰유합시간병계산유합면적비솔,정기취창면조직행CD31、파형단백면역조화검측,병이Hoechst33342형광표기MEFs,시종기전귀.결과 여FBs조상비,MEFs조적IL-6분비경가왕성,이TGF-β1분비량소(P＜0.05).실험조창면유합쾌(P＜0.05),미혈관밀도고(P＜0.05),창면중FBs배렬유서,이식적MEFs수시간감소.결론 MEFs삼유구건가가속창면유합,감경반흔형성,기궤제가능여배태세포대창면유합적유도유관.
Objective To investigate the application and mechanism of tissue-engineered skin with mouse embryonic fibroblasts(MEFs)for the full-thickness skin defects on mice.Methods The MEFs and fibroblasts were cultured and seeded in scaffold made of rat tail collage.ELISA method was used for detection of secretory function.The full-thickness skin defects were created on mice and covered by MEFsscaffold complex(experimental group),or FBs-scaffold complex(control group 1),or scaffold only(control group 2).The process of wound healing was evaluated by observation of the re-epithelization rate.Microvessal density(MVD)and vimentin within the wound sites were also detected with immunohistochemistry staining technique to describe the characteristics of wound healing.Hoechst 33342 staining was performed to trace MEFs'fate.Results MEFs scaffold group had higher level secretion of IL-6 and lower of TGF-β1 than FBs scaffold group(P＜0.05).Compared with wounds in control groups,the wounds in MEFs group healed markedly fast(P＜0.05)and the MVD was significantly higher(P＜0.05).The fibroblasts in the wounds of MEFs group were arranged regularly and the MEFs decreased during the healing process.Conclusions The MEFs-scaffold complex can promote wound healing with less scar formation.MEFs may have an inducing effect on the wound healing.
