CAJ | 학술논문

目的对影响青蒿丛生芽诱导因素进行基础性研究.方法把常规的植物组织培养技术应用于调控青蒿中次生代谢产物青蒿素的生物合成代谢.结果青蒿的基因型,激素和基本培养基对丛生芽的发生有显著影响,而光强在1000～6000lx和温度在20℃～30℃对丛生芽的发生影响不大;在5种基因型的青蒿中,025丛生芽的诱导率最高;诱导丛生芽的激素组合是6-BA 2.0mg/L和NAA 0.15mg/L;另外,离子在青蒿丛生芽的诱导和青蒿素的生物合成过程中起着非常重要的作用.结论组织培养条件下,青蒿丛生芽的诱导及青蒿素的生物合成可以通过理化因子有效地进行调控.
목적대영향청호총생아유도인소진행기출성연구.방법파상규적식물조직배양기술응용우조공청호중차생대사산물청호소적생물합성대사.결과청호적기인형,격소화기본배양기대총생아적발생유현저영향,이광강재1000～6000lx화온도재20℃～30℃대총생아적발생영향불대;재5충기인형적청호중,025총생아적유도솔최고;유도총생아적격소조합시6-BA 2.0mg/L화NAA 0.15mg/L;령외,리자재청호총생아적유도화청호소적생물합성과정중기착비상중요적작용.결론조직배양조건하,청호총생아적유도급청호소적생물합성가이통과이화인자유효지진행조공.
Object To study the factors affecting the induction of shoot cluster of Artemisia annua.Methods Conventional plant tissue culture technology was applied to regulate the biosynthetic metabolism of secondary metabolism product,artemisinin of A.annua.Results Effects of genotype,hormones,basal medium on the induction of A.annua shoot cluster were notable,while that of light intensity ranging between 1000-6000 lx and temperature ranging between 20 ℃-30 ℃ were not.Among the five A.annua strains used,the induction ratio of strain 025 was the best.The optimal concentration for induction of shoot cluster was 6-BA 2.0 mg/L and NAA 0.15 mg/L.Ions were also found to be very important in the shoot cluster induction and the biosynthesis of artemisnin.Conclusion Effective regulation of induction of A.annua shoot cluster and artemisinin biosynthesis can be carried out via the regulation of physical and chemical factors.