CAJ | 학술논문

目的通过检测大鼠宫颈基质金属蛋白2(MMP-2)、基质金属蛋白8(MMP-8)的动态表达,探讨胶原降解与宫颈成熟的关系.方法清洁级大鼠分为未孕动情间期、妊娠10d、妊娠16d、妊娠19d和分娩后即刻组,每组各6只.宫颈称湿重.HE 常规染色显示宫颈大体结构,VG染色进行胶原纤维和肌纤维显色,免疫组织化学染色显示MMP-2、MMP-8.结果 (1)HE染色显示大鼠宫颈主要由大量纤维(胶原纤维和肌纤维)组成,分娩后即刻组宫颈间质见少量中性粒细胞.其余组未见中性粒细胞.(2)临产前宫颈湿重已有增加,至分娩后即刻增加更明显.(3)宫颈纤维组织由胶原纤维和平滑肌纤维组成,所占比例无明显差别.分娩后即刻组胶原纤维和肌纤维排列较非分娩时松散.(4)MMP-2(棕染)在所有组别间质均有表达,鳞状上皮无表达,胶原纤维、平滑肌纤维、上皮及少量血管壁呈阳性表达;妊娠19d和分娩后即刻组阳性表达的密度较其他组增强.(5)分娩后即刻组MMP-8染色见间质散在中性粒细胞棕染.其他组的组织或细胞均未见阳性表达.结论宫颈成熟后,官颈纤维组织松散、断裂,中性粒细胞由外源性血管浸润到宫颈间质,这一变化在分娩时尤其明显;MMP-2、MMP-8这两种不同来源的基质金属蛋白酶共同降解了宫颈纤维,导致宫颈软化、扩张.
목적 통과검측대서궁경기질금속단백2(MMP-2)、기질금속단백8(MMP-8)적동태표체,탐토효원강해여궁경성숙적관계.방법 청길급대서분위미잉동정간기、임신10d、임신16d、임신19d화분면후즉각조,매조각6지.궁경칭습중.HE 상규염색현시궁경대체결구,VG염색진행효원섬유화기섬유현색,면역조직화학염색현시MMP-2、MMP-8.결과 (1)HE염색현시대서궁경주요유대량섬유(효원섬유화기섬유)조성,분면후즉각조궁경간질견소량중성립세포.기여조미견중성립세포.(2)임산전궁경습중이유증가,지분면후즉각증가경명현.(3)궁경섬유조직유효원섬유화평활기섬유조성,소점비례무명현차별.분면후즉각조효원섬유화기섬유배렬교비분면시송산.(4)MMP-2(종염)재소유조별간질균유표체,린상상피무표체,효원섬유、평활기섬유、상피급소량혈관벽정양성표체;임신19d화분면후즉각조양성표체적밀도교기타조증강.(5)분면후즉각조MMP-8염색견간질산재중성립세포종염.기타조적조직혹세포균미견양성표체.결론 궁경성숙후,관경섬유조직송산、단렬,중성립세포유외원성혈관침윤도궁경간질,저일변화재분면시우기명현;MMP-2、MMP-8저량충불동래원적기질금속단백매공동강해료궁경섬유,도치궁경연화、확장.
Objective To elucidate the relationship between collagen degradation and cervical ripening by detecting dynamic expressions of matrix metalloproteinases 2 (MMP-2) and 8 (MMP-8) in rat cervix. Method SPF rats were divided into 5 groups (n=6), namely non-pregnancy estrus interval group, gestational days 10, 16, and 19 groups, and immediately postpartum group. The wet weight of the cervix was measured and HE staining was used to display the general structure of the cervix. VG staining was applied to visualize the collagen fibers and muscular fibers. Immunohistochemistry was performed to observe the expressions of MMP-2 and MMP-8 in the cervix. Results HE staining showed that the rat uterine cervix consisted mainly of ?broblasts and ?brous connective tissues. A small quantity of neutrophils could be seen in the cervix stroma of the rats immediately after immediately parturition, but not at the other time points. The wet weight of the antepartum cervix had increased, and a more obvious increase was seen in the wet weight of the cervix immediately after parturition. The collagen fibers of the cervix consisted of collagen fibers and smooth muscle fibers, and their proportions showed no significant variation at the time points around the parturition. Immediately after parturition, the collagen fibers and muscular fibers in the cervix became loosened as compared with that before parturition. MMP-2 expression was found in the cervical stroma but not in the squamous epithelium in nonpregnancy, term pregnancy, and immediately after parturition; the smooth muscle cells, vascular wall, and stromal ?broblasts showed positive expression of MMP-2. Enhanced intensity of MMP-2 staining was seen in term pregnancy and postpartum group in comparison with that in the other groups. MMP-8 expression was observed in the cervix of rats immediately after parturition, with scattered neutrophils positive for MMP-8 spotted in the stroma of the ripened cervix. MMP-8 expression was not detected in the other groups. Conclusion Ripened cervical fibrous tissue becomes loose and broken, and cervical ripening is accompanied by infiltration of neutrophils from exogenous vessels. These changes are particularly evident after parturition. MMP-2 and MMP-8 cooperate to degrade the cervical fibers, leading to cervical softening and expansion.