CAJ | 학술논문

目的研究X-性染色体连锁凋亡抑制蛋白(XIAP)在口腔鳞癌细胞Tca8113中的表达水平,并探讨XIAP表达与Tca8113细胞对化疗药物耐药性之间的关系.方法用平阳霉素(PYM)间歇性加药,逐步递增剂量,采用甲基噻唑基四唑(MTT)法检测药物处理前后细胞对PYM的敏感性,逆转录聚合酶链反应(RT-PCR)分别检测XIAP在药物处理前后的各Tca8113细胞组中的表达变化,并探讨XIAP与细胞耐药性的关系.结果Tca8113细胞在PYM间断作用下产生耐受,Tea81 13-1-10组、Tca8113-10-10组耐药细胞对PYM的半数有效浓度(IC_(50)分别为(12.758±0.030)、(18.986±0.150)μg·mL~(-1).Tca8113-1-20、Tca8113-10-20组耐药细胞对PYM的IC_(50)分别为(26.302±0.072)、(35.294±0.115)μg·mL~(-1).XAIP的表达水平与细胞对PYM的耐药有相关性(P<0.01).结论XIAP在口腔鳞癌中的表达水平升高,可能与鳞癌的化疗耐药性有关,这可作为口腔鳞癌基因治疗的一个新靶点.
목적연구X-성염색체련쇄조망억제단백(XIAP)재구강린암세포Tca8113중적표체수평,병탐토XIAP표체여Tca8113세포대화료약물내약성지간적관계.방법용평양매소(PYM)간헐성가약,축보체증제량,채용갑기새서기사서(MTT)법검측약물처리전후세포대PYM적민감성,역전록취합매련반응(RT-PCR)분별검측XIAP재약물처리전후적각Tca8113세포조중적표체변화,병탐토XIAP여세포내약성적관계.결과Tca8113세포재PYM간단작용하산생내수,Tea81 13-1-10조、Tca8113-10-10조내약세포대PYM적반수유효농도(IC_(50)분별위(12.758±0.030)、(18.986±0.150)μg·mL~(-1).Tca8113-1-20、Tca8113-10-20조내약세포대PYM적IC_(50)분별위(26.302±0.072)、(35.294±0.115)μg·mL~(-1).XAIP적표체수평여세포대PYM적내약유상관성(P<0.01).결론XIAP재구강린암중적표체수평승고,가능여린암적화료내약성유관,저가작위구강린암기인치료적일개신파점.
Objective To explore the expression of X-linked inhibitor of apoptosis protein (XIAP) in Tca8113 cell, and to investigate its relationship to the chemoresistance. Methods The Tca8113 cell line was cultured by IMDM and the concentration of Pingyangmycin (PYM) added to Tca8113 cell line was increased gradually and con tinually, which was to induce the PYM-resistance in Tca8113 cell line. The sensitivity of Tca8113 cell to PYM and expression of XIAP were measured with methyl thiazolyl tetrazolium (MTT) chromatometry and reverse transcription -polymerase chain raction (RT-PCR). The XIAP level in the cells and its chemoresistance to PYM were analyzed by linear regressioa Results The IC_(50) of Tca8113-l-10 group and Tca8113-10-10 group were(12.758±0.030), (18.986± 0.150) μg·mL~(-1) respectively. The IC_(50) of Tca8113-l-20 group and Tca8113-10-20 group increased to(26.302±0.072), O5.294±0.115) μg·mL~(-1) respectively. There was a relation between XIAP and the drug-resistance in Tca8113 cell. Conclusion XIAP may play an important role in the chemoresistance which might serve as a new therapeutic target for oral squamous cell carcinoma.