时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
532-534
,共3页
李静%陈长勋%高阳%焦亚斌%吴喜民%王瑞%吴迎春%李医明
李靜%陳長勛%高暘%焦亞斌%吳喜民%王瑞%吳迎春%李醫明
리정%진장훈%고양%초아빈%오희민%왕서%오영춘%리의명
玄参提取物%动脉硬化%抗炎%血脂
玄參提取物%動脈硬化%抗炎%血脂
현삼제취물%동맥경화%항염%혈지
Radix Scrophulariae extracts%Atherosclerosis%Anti-inflammation%Serum lipid
目的 观察玄参提取物对大鼠动脉硬化及其炎症相关细胞因子的影响.方法 将大鼠随机分为正常组、模型组、玄参提取物低剂量组、玄参提取物高剂量组和布洛芬组.模型组及各给药组采用一次性腹腔注射维生素D_3 60万单位/kg,同时每天灌服10 ml/kg脂肪乳的方法制备大鼠动脉硬化模型.玄参低剂量组和高剂量组分别每天灌服玄参提取物20.5 mg·kg~(-1)·d~(-1)和41.0 mg·kg~(-1).D~(-1),布洛芬组每天灌服布洛芬混悬液144 mg·kg~(-1)·d~(-1),正常组和模型组每天灌服等量蒸馏水.给药70 d后处死大鼠,对血脂、TNF-α,IL-1β,IL-6,IL-10 及NF-кB进行测定,并观察动脉的病变情况.结果 玄参提取物可明显降低模型大鼠胆固醇和低密度脂蛋白的水平,提高高密度脂蛋白与低密度脂蛋白的比值,降低炎症因子TNF-α,IL-1β,IL-6的浓度,提高抗炎因子IL-10的浓度,抑制动脉NF-кB过量表达,抑制模型大鼠动脉壁中膜的增厚.结论 玄参提取物可通过抗炎和降血脂而发挥一定的抗动脉硬化的作用.
目的 觀察玄參提取物對大鼠動脈硬化及其炎癥相關細胞因子的影響.方法 將大鼠隨機分為正常組、模型組、玄參提取物低劑量組、玄參提取物高劑量組和佈洛芬組.模型組及各給藥組採用一次性腹腔註射維生素D_3 60萬單位/kg,同時每天灌服10 ml/kg脂肪乳的方法製備大鼠動脈硬化模型.玄參低劑量組和高劑量組分彆每天灌服玄參提取物20.5 mg·kg~(-1)·d~(-1)和41.0 mg·kg~(-1).D~(-1),佈洛芬組每天灌服佈洛芬混懸液144 mg·kg~(-1)·d~(-1),正常組和模型組每天灌服等量蒸餾水.給藥70 d後處死大鼠,對血脂、TNF-α,IL-1β,IL-6,IL-10 及NF-кB進行測定,併觀察動脈的病變情況.結果 玄參提取物可明顯降低模型大鼠膽固醇和低密度脂蛋白的水平,提高高密度脂蛋白與低密度脂蛋白的比值,降低炎癥因子TNF-α,IL-1β,IL-6的濃度,提高抗炎因子IL-10的濃度,抑製動脈NF-кB過量錶達,抑製模型大鼠動脈壁中膜的增厚.結論 玄參提取物可通過抗炎和降血脂而髮揮一定的抗動脈硬化的作用.
목적 관찰현삼제취물대대서동맥경화급기염증상관세포인자적영향.방법 장대서수궤분위정상조、모형조、현삼제취물저제량조、현삼제취물고제량조화포락분조.모형조급각급약조채용일차성복강주사유생소D_3 60만단위/kg,동시매천관복10 ml/kg지방유적방법제비대서동맥경화모형.현삼저제량조화고제량조분별매천관복현삼제취물20.5 mg·kg~(-1)·d~(-1)화41.0 mg·kg~(-1).D~(-1),포락분조매천관복포락분혼현액144 mg·kg~(-1)·d~(-1),정상조화모형조매천관복등량증류수.급약70 d후처사대서,대혈지、TNF-α,IL-1β,IL-6,IL-10 급NF-кB진행측정,병관찰동맥적병변정황.결과 현삼제취물가명현강저모형대서담고순화저밀도지단백적수평,제고고밀도지단백여저밀도지단백적비치,강저염증인자TNF-α,IL-1β,IL-6적농도,제고항염인자IL-10적농도,억제동맥NF-кB과량표체,억제모형대서동맥벽중막적증후.결론 현삼제취물가통과항염화강혈지이발휘일정적항동맥경화적작용.
Objective To study the effect of extract from Radix Scrophulariae (RS) against atherosclerosis and the relative mechanisms in rats.Methods Rats were randomly divided into 5 groups. They were the conrtol , model,low and high dose of RS extract and ibuprofen groups.The model rats were intraperitoneally injected with 600 000 IU·kg-1 vitamine D_3 and intragastrically administrated high-fat emulsion 10 ml· kg~(-1)·d~(-1) for 70 days.At the same time, the model rats treated with drugs were orally given 20.5mg·kg~(-1).D~(-1),41.0 mg·kg~(-1)·d~(-1) RS extract and 144.0 mg·kg~(-1)·d~(-1) ibuprofen suspension respectively.After 70days,the rats were killed, and the serum levels of TC,TG,LDL,HDL/LDL,TNF-α,IL-1β,IL-6,IL-10,the expression of NF-кB in rat aorta and the pathological change of aorta were measured.Results RS extract could lower the serum levels of TC,LDL-C, TNF-α,IL-1β and IL-6, increase the level of IL-10 and the ratio of HDL/LDL,inhibit the expression of NF-кB and reduce the thickness of the artery wall.Conclusion RS extract has anti-atherescle ratic effect.The mechanism may be related with inhibiting inflammation and lowering serum lipid.
