CAJ | 학술논문

目的探讨蓖麻毒素温敏型凝胶经皮瘤内注射治疗实验性肝癌的有效性和可行性.方法建立裸鼠皮下移植肝癌模型32只,随机分成4组,分别经皮瘤内注射生理盐水、空白凝胶、游离蓖麻毒素和蓖麻毒素温敏型凝胶,每周超声测量肿瘤体积,第7、14 d每组处死裸鼠1只行肿瘤组织病理检查,余裸鼠记录生存时间.结果游离蓖麻毒素组治疗后7、14、21 d肿瘤体积均小于生理盐水对照组(P<0.01),生命延长率为36.8%;蓖麻毒素温敏型凝胶组治疗后第5 d肝癌组织明显坏死,第3周肿瘤消退,病理检查见肿瘤组织大片坏死,伴大量炎性细胞浸润,裸鼠在生存时间的最终观察点均未见死亡.结论蓖麻毒素温敏型凝胶瘤内注射可彻底消融灭活肝癌,安全高效且操作简便.
목적 탐토비마독소온민형응효경피류내주사치료실험성간암적유효성화가행성.방법 건립라서피하이식간암모형32지,수궤분성4조,분별경피류내주사생리염수、공백응효、유리비마독소화비마독소온민형응효,매주초성측량종류체적,제7、14 d매조처사라서1지행종류조직병리검사,여라서기록생존시간.결과 유리비마독소조치료후7、14、21 d종류체적균소우생리염수대조조(P<0.01),생명연장솔위36.8%;비마독소온민형응효조치료후제5 d간암조직명현배사,제3주종류소퇴,병리검사견종류조직대편배사,반대량염성세포침윤,라서재생존시간적최종관찰점균미견사망.결론 비마독소온민형응효류내주사가철저소융멸활간암,안전고효차조작간편.
Objective To evaluate the effectiveness and feasibility of riein-loaded thermosenaitive hydrogel in percutaneous intratumoral injection for treatment of hepatic carcinoma. Methods BALB/c-nu mice were inoculated subcutaneously in the right flank with human liver cancer cell line HepG2. A total of 32 male mice bearing subcutaneous hepatic carcinoma (7-10mm in diameter) were randomized into 4 groups. Each group of mice were administered percutaneous intratumoral injection of different agents as follows: salin, thermosensitive hydrogel,ricin, ricin-loaded thermosensitive hydrogel. The tumor volume was totally measured 3 times by high frequency ultrasound once a week. One mouse of each group was sacrificed to perform tumor histopathological examination on 7th day, 14th day, respectively, and survival time of the remaining mice was recorded. Results After intratumoral therapy,tumor volume in ricin group was smaller than that of saline group on 7th day, 14th day and 21st day( P<0.01 ),and mice got a life-prolonging rate of 36.8%. In ricin-loaded thermosensitive hydrogel group,obvious necrosis occurred in tumors treated since 5th day, and the tumor almost regressed on 21st day. Histopathological assay showed serious necrosis with a large number of leucocyte infiltration in tumor. All the remaining mice treated with riein-loaded thermosensitive hydrogel survived at the end of experiment sans tumor recurrence. Conclusions Percutaneous intratutnoral injection of ricin-loaded thermosensitive hydrogel could completely ablate hepatic carcinoma,which may provide an effective and feasible method for tumor ablation therapy.