CAJ | 학술논문

目的了解中国6省蜱中莱姆病螺旋体携带情况.方法在6省各选取2个采样点捕蜱,采用病原分离培养和巢式PCR方法对蜱进行莱姆病螺旋体检测,通过基因测序确定分型.结果 6省共采集到2200余只蜱,约1000只蜱用于病原分离培养,从吉林省长白县全沟硬蜱标本中分离到13株螺旋体,从贵州省道真粒形硬蜱标本中分离到9株螺旋体.1255只蜱用于PCR检测,从6省的蜱标本中检测到莱姆病螺旋体特异片段,其中吉林省(长白县27.08%、通化县20.41%)、青海省(互助县25.06%、黄南县21.11%)和贵州省(道真县25.63%)蜱中莱姆病螺旋体阳性率较高,山西省(垣曲县4.72%、交城县3.64%)蜱中莱姆病螺旋体阳性率较低.通过序列同源性分析确定吉林、青海、甘肃和山西省蜱中莱姆病螺旋体基因型均为Borrelia garinii.贵州、湖南省的基因型均为Borrelia valaisiana.结论 6省蜱中均带有莱姆病螺旋体,且带菌率有差异;山西省蜱中存在Borrelia ganmi型莱姆病螺旋体,湖南省蜱中存在Borrelia valaisiana型莱姆病螺旋体.
목적 료해중국6성비중래모병라선체휴대정황.방법 재6성각선취2개채양점포비,채용병원분리배양화소식PCR방법대비진행래모병라선체검측,통과기인측서학정분형.결과 6성공채집도2200여지비,약1000지비용우병원분리배양,종길림성장백현전구경비표본중분리도13주라선체,종귀주성도진립형경비표본중분리도9주라선체.1255지비용우PCR검측,종6성적비표본중검측도래모병라선체특이편단,기중길림성(장백현27.08%、통화현20.41%)、청해성(호조현25.06%、황남현21.11%)화귀주성(도진현25.63%)비중래모병라선체양성솔교고,산서성(원곡현4.72%、교성현3.64%)비중래모병라선체양성솔교저.통과서렬동원성분석학정길림、청해、감숙화산서성비중래모병라선체기인형균위Borrelia garinii.귀주、호남성적기인형균위Borrelia valaisiana.결론 6성비중균대유래모병라선체,차대균솔유차이;산서성비중존재Borrelia ganmi형래모병라선체,호남성비중존재Borrelia valaisiana형래모병라선체.
Objective To understand the carrying status of Borrelia burgdorferi in ticks from the mountain areas from six representative provinces, including Jilin, Shanxi, Gansu, Qinghai,Guizhou and Hunan in China. Methods Flagging and trapping methods were used to collect ticks in forest area and culture medium was used to isolate the pathogen. Nested-PCR was used to detect the gem-carrying rate of ticks. Results More than 2200 ticks from six representative provinces were collected and 1000 ticks were used to isolate the pathogen. 13 Lyme disease spirochetes from ixodes persulcatus in Changbai, Jilin province and 9 Lyme disease spirochetes from ixodes granulatus in Daozhen, Guizhou province were identified. There were 1255 ticks used for PCR testing. Specific fragments of the Borrelia burgdorferi in ticks were found from the six representative provinces in China. The carrier rate was higher in Jilin (Changbai 27.08%, Tonghua 20.41% ), Qinghai (Huzhu 25.06%, Huangnan 21.11%)and Guizhou (Daozhen 25.63% ), than in Shanxi (Yuanqu 4.72%,Jiaocheng 3.64% ). Result from the sequence analysis showed that the genotype belong to Borrelia garinii in Jilin, Qinghai, Gansu, Shanxi provinces but Borrelia valaisiana in Guizhou and Hunan provinces. Conclusion Our data showed that there existed Lyme disease spirochetes in all the six representative provinces in China, but the carriying rates of ticks were different. Borrelia garinii was found in Shanxi province, and Borrelia valaisiana in Hunan province.