中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2009年
1期
31-34
,共4页
疱疹,单纯%脑炎%早期诊断%酶联免疫吸附测定%疱疹病毒1型,人%免疫球蛋白M
皰疹,單純%腦炎%早期診斷%酶聯免疫吸附測定%皰疹病毒1型,人%免疫毬蛋白M
포진,단순%뇌염%조기진단%매련면역흡부측정%포진병독1형,인%면역구단백M
Herpes simplex%Encephalitis%Early diagnosis%Enzyme-linked immunosorbent assay%Herpesvirus 1,human%Immunoglobulin M
目的 观察酶联免疫斑点法(ELISPOT)检测抗1型单纯疱疹病毒IgM(抗-HSV-1 IgM)分泌细胞在单纯疱疹病毒性脑炎(HSV-1脑炎)早期诊断中的作用并探讨其价值.方法 采用ELISPOT法和ELISA法分别检测患者脑脊液中的抗-HSV-1 IgM分泌细胞和抗-HSV-1 IgM,并对HSV-1腩炎23例、临床对照组40例进行回顾性分析,统计学处理采用Fisher精确检验法.结果 ELISPOT法检测HSV-1脑炎组9例患者2周内脑脊液中抗-HSV-1 IgM分泌细胞,8例阳性.敏感度为88.9%;临床对照组检测16例,15例阴性,特异度为93.8%.ELISA法榆测HSV-1脑炎组12例患者脑脊液2周内抗-HSV-1 IgM,2例阳件,敏感度为16.6%;临床对照组检测17例,15例阴性,特异度为88.2%.两种方法敏感度比较差异有统汁学意义(P<0.01).结论 应用ELISPOT法检测HSV-1脑炎患者脑脊液中抗-HSV-1 IgM分泌细胞优于 ELISA方法检测抗-HSV-1 IgM,具有更好的早期诊断意义.
目的 觀察酶聯免疫斑點法(ELISPOT)檢測抗1型單純皰疹病毒IgM(抗-HSV-1 IgM)分泌細胞在單純皰疹病毒性腦炎(HSV-1腦炎)早期診斷中的作用併探討其價值.方法 採用ELISPOT法和ELISA法分彆檢測患者腦脊液中的抗-HSV-1 IgM分泌細胞和抗-HSV-1 IgM,併對HSV-1腩炎23例、臨床對照組40例進行迴顧性分析,統計學處理採用Fisher精確檢驗法.結果 ELISPOT法檢測HSV-1腦炎組9例患者2週內腦脊液中抗-HSV-1 IgM分泌細胞,8例暘性.敏感度為88.9%;臨床對照組檢測16例,15例陰性,特異度為93.8%.ELISA法榆測HSV-1腦炎組12例患者腦脊液2週內抗-HSV-1 IgM,2例暘件,敏感度為16.6%;臨床對照組檢測17例,15例陰性,特異度為88.2%.兩種方法敏感度比較差異有統汁學意義(P<0.01).結論 應用ELISPOT法檢測HSV-1腦炎患者腦脊液中抗-HSV-1 IgM分泌細胞優于 ELISA方法檢測抗-HSV-1 IgM,具有更好的早期診斷意義.
목적 관찰매련면역반점법(ELISPOT)검측항1형단순포진병독IgM(항-HSV-1 IgM)분비세포재단순포진병독성뇌염(HSV-1뇌염)조기진단중적작용병탐토기개치.방법 채용ELISPOT법화ELISA법분별검측환자뇌척액중적항-HSV-1 IgM분비세포화항-HSV-1 IgM,병대HSV-1남염23례、림상대조조40례진행회고성분석,통계학처리채용Fisher정학검험법.결과 ELISPOT법검측HSV-1뇌염조9례환자2주내뇌척액중항-HSV-1 IgM분비세포,8례양성.민감도위88.9%;림상대조조검측16례,15례음성,특이도위93.8%.ELISA법유측HSV-1뇌염조12례환자뇌척액2주내항-HSV-1 IgM,2례양건,민감도위16.6%;림상대조조검측17례,15례음성,특이도위88.2%.량충방법민감도비교차이유통즙학의의(P<0.01).결론 응용ELISPOT법검측HSV-1뇌염환자뇌척액중항-HSV-1 IgM분비세포우우 ELISA방법검측항-HSV-1 IgM,구유경호적조기진단의의.
Objective To investigate the role of anti-herpes ximplex virus (HSV)-1 IgM secreting cells detection assay in early diagnosis of herpes simplex encephalitis. Methods Twenty-three herpes simplex encephalitis cases and 40 control cases were included in this study. Anti-HSV-1 IgM secreting cells and anti-HSV-1 IgM were retrospectively tested in the patients' cerebrospinal fluid by enzyme-linked immunosorbent spot (ELISPOT) and enzyme-linked immunosorbent assay (ELISA), respectively. The data analysis was performed by using Fisher Exact Test. Results Using ELISPOT method for detection of 9 HSV-1 encephalitis patients' and 16 clinical control cases anti-HSV-1 IgM secreting cells within two weeks after disease onset, the sensitivity of ELISPOT for detecting anti HSV-1 IgM secreting cells in the cerebrospinal fluid was 88.9% (8/9) and the specificity was 93.8%(15/16). On the other hand, the sensitivity of ELISA for detecting anti-HSV-1 IgM in ccrebrospinal fluid was 16.6% (2/12) and the specificity was 88.2% (15/17) when using ELISA method for detection of 12 HSV-1 encephalitis patients' and 17 clinical control cases's anti-HSV-1 IgM secreting cells. The sensitivities of the two methods were statistically different (P<0.01). Conclusion Compared to ELISA, ELISPOT for detecting the anti-HSV-1 IgM secreting cells in cerehrospinal fluid is a more sensitive method for early diagnosing herpes simplex encephalitis.
