CAJ | 학술논문

目的通过建立慢性酒精中毒致大鼠学习记忆障碍模型,定量分析大鼠海马各亚区磷酸化环磷酸腺苷反应单元结合蛋白(p-CREB)的分布与表达.方法 60只成年雄性SD大鼠随机平均分为4组:对照组、低浓度染毒组(LAA组)、中剂浓度染毒组(MAA组)、高浓度染毒组(HAA组).对照组以生理盐水灌胃,LAA组、MAA组和HAA组分别按照每天每只5ml/kg、10ml/kg、15ml/kg标准用55%酒精灌胃造模.通过Morris水迷宫检测大鼠学习记忆损伤情况,采用免疫组织化学SABC染色法检测海马CA1、CA3区及齿状回p-CREB的分布及表达.在Olympus显微镜及图像分析系统下,测定海马p-CREB阳性细胞数.结果慢性酒精中毒后LAA组、MAA组和HAA组大鼠海马各亚区p-CREB免疫阳性细胞数量逐渐减少.对照组海马p-CREB 阳性反应物分布较多,以CA3和CA1区着色最深,齿状回颗粒细胞层未见表达;LAA组、MAA组和HAA组海马CA1区和CA3区p-CREB免疫阳性细胞数[分别为(85.45±4.32)个,(74.42±8.94)个,(73.56±7.64)个和(245.29±15.36)个,(206.90±15.37)个,(201.96±11.37)个]与对照组海马CA1区和CA3区p-CREB免疫阳性细胞数[分别为(90.54± 6.38)个,(273.79±16.87)个]比差异有统计学意义( P <0.05),MAA组和HAA组海马CA1区和CA3区p-CREB免疫阳性细胞数与LAA组比差异有统计学意义( P <0.05).结论高浓度白酒灌胃6周,大鼠学习记忆功能有不同程度损伤,各染毒组大鼠海马各亚区p-CREB有不同程度表达,提示p-CREB在大鼠慢性酒精中毒学习记忆障碍中可能起重要作用.
목적 통과건립만성주정중독치대서학습기억장애모형,정량분석대서해마각아구린산화배린산선감반응단원결합단백(p-CREB)적분포여표체.방법 60지성년웅성SD대서수궤평균분위4조:대조조、저농도염독조(LAA조)、중제농도염독조(MAA조)、고농도염독조(HAA조).대조조이생리염수관위,LAA조、MAA조화HAA조분별안조매천매지5ml/kg、10ml/kg、15ml/kg표준용55%주정관위조모.통과Morris수미궁검측대서학습기억손상정황,채용면역조직화학SABC염색법검측해마CA1、CA3구급치상회p-CREB적분포급표체.재Olympus현미경급도상분석계통하,측정해마p-CREB양성세포수.결과 만성주정중독후LAA조、MAA조화HAA조대서해마각아구p-CREB면역양성세포수량축점감소.대조조해마p-CREB 양성반응물분포교다,이CA3화CA1구착색최심,치상회과립세포층미견표체;LAA조、MAA조화HAA조해마CA1구화CA3구p-CREB면역양성세포수[분별위(85.45±4.32)개,(74.42±8.94)개,(73.56±7.64)개화(245.29±15.36)개,(206.90±15.37)개,(201.96±11.37)개]여대조조해마CA1구화CA3구p-CREB면역양성세포수[분별위(90.54± 6.38)개,(273.79±16.87)개]비차이유통계학의의( P <0.05),MAA조화HAA조해마CA1구화CA3구p-CREB면역양성세포수여LAA조비차이유통계학의의( P <0.05).결론 고농도백주관위6주,대서학습기억공능유불동정도손상,각염독조대서해마각아구p-CREB유불동정도표체,제시p-CREB재대서만성주정중독학습기억장애중가능기중요작용.
Objective Through establishing the rat model of learning and memory disorder,This study is to detect the relative densities of p-CREB distribution and expression in hippocampus,and explore the effects of p-CREB on learning and memory disorder of rat chronic alcoholism. Methods 60 adult male SD rats were randomly divided to 4 groups:control group,low alcohol administration group(LAA group),moderate alcohol administration group(MAA group),high alcohol administration group(HAA group). Control group were administrated by normal saline,different dosage of 55% alcohol (5 ml·kg-1·d-1,10 ml·kg-1·d-1 and 15 ml·kg-1·d-1) were respectively administrated to LAA,MAA and HAA group. Morris water maze was used to detect the changes of learning and memory. Distribution and expression of p-CREB in hippocampus CA1,CA3 region and dentate gyrus were observed with immunohistochemical SABC staining. The p-CREB-immunoreactive cells numbers in hippocampal formation were detected by Olympus microscope and image analysis system. Results The impairment of learning and memory was unequal.The p-CREB-immunoreactive cells in control group hippocampus were distributed more than in LAA group,MAA group and HAA group hippocampus,and the colour of p-CREB-immunoreactive cells was deeper in CA1 and CA3 region,but p-CREB-immunoreactive cells were not observed in dentate gyrus.The numbers of p-CREB-immunoreactive cells in CA1,CA3 region of LAA group,MAA group and HAA group decreased obviously after alcohol intragastric administration [CA1:85.45±4.32,74.42±8.94,73.56±7.64; CA3:245.29±15.36,206.90±15.37,201.96±11.37] compared with control group [CA1:90.54± 6.38; CA3:273.79±16.87 ]( P <0.05). Conclusion After 6 weeks' high ethanol administration,the impairments of learning and memory were unequal,the expression of p-CREB was observed in all groups,but there are some differences.These suggest that p-CREB might play an important role in the rat learning and memory disorder induced by chronic alcoholism.