中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
4期
425-427
,共3页
姜明%郑毅雄%唐湘莲%孟立峰%陈力
薑明%鄭毅雄%唐湘蓮%孟立峰%陳力
강명%정의웅%당상련%맹립봉%진력
胃癌%趋化因子受体%CXCR4%CCR7
胃癌%趨化因子受體%CXCR4%CCR7
위암%추화인자수체%CXCR4%CCR7
Gastric carcinoma%Chemokine receptors%CXCR4%CCR7
目的 观察趋化因子受体CXCR4及CCR7在不同侵袭能力人胃癌细胞株中的差异表达.方法 逆转录-聚合酶链反应(RT-PCR)及Western blot分析CXCR4及CCR7在人胃癌细胞株MGC803、AGS、BGC823、SGC7901的表达;体外侵袭实验测定4株胃癌细胞的侵袭能力.结果 MGC803、AGS、BGC823、SGC7901中CXCR4 mRNA相对表达量分别为:1.2556±0.1384、0.7943±0.0913、0.4749±0.0744、0.2463±0.0344,CCR7 mRNA相对表达量分别为:0.6071±0.1404、0.5355±0.0750、0.2549±0.0522、0.2466±0.0342,CXCR4及CCR7蛋白表达趋势同其相对应的基因表达趋势基本一致.4株胃癌细胞侵袭实验测定的侵袭细胞数分别为:400.0±18.2、310.0±4.0、110.0±13.9、85.0±9.5.CXCR4在不同侵袭能力的胃癌细胞株中存在差异性表达(P<0.05).结论 CXCR4的表达与胃癌细胞侵袭能力成正相关,CCR7的表达与胃癌细胞侵袭能力无明显相关.
目的 觀察趨化因子受體CXCR4及CCR7在不同侵襲能力人胃癌細胞株中的差異錶達.方法 逆轉錄-聚閤酶鏈反應(RT-PCR)及Western blot分析CXCR4及CCR7在人胃癌細胞株MGC803、AGS、BGC823、SGC7901的錶達;體外侵襲實驗測定4株胃癌細胞的侵襲能力.結果 MGC803、AGS、BGC823、SGC7901中CXCR4 mRNA相對錶達量分彆為:1.2556±0.1384、0.7943±0.0913、0.4749±0.0744、0.2463±0.0344,CCR7 mRNA相對錶達量分彆為:0.6071±0.1404、0.5355±0.0750、0.2549±0.0522、0.2466±0.0342,CXCR4及CCR7蛋白錶達趨勢同其相對應的基因錶達趨勢基本一緻.4株胃癌細胞侵襲實驗測定的侵襲細胞數分彆為:400.0±18.2、310.0±4.0、110.0±13.9、85.0±9.5.CXCR4在不同侵襲能力的胃癌細胞株中存在差異性錶達(P<0.05).結論 CXCR4的錶達與胃癌細胞侵襲能力成正相關,CCR7的錶達與胃癌細胞侵襲能力無明顯相關.
목적 관찰추화인자수체CXCR4급CCR7재불동침습능력인위암세포주중적차이표체.방법 역전록-취합매련반응(RT-PCR)급Western blot분석CXCR4급CCR7재인위암세포주MGC803、AGS、BGC823、SGC7901적표체;체외침습실험측정4주위암세포적침습능력.결과 MGC803、AGS、BGC823、SGC7901중CXCR4 mRNA상대표체량분별위:1.2556±0.1384、0.7943±0.0913、0.4749±0.0744、0.2463±0.0344,CCR7 mRNA상대표체량분별위:0.6071±0.1404、0.5355±0.0750、0.2549±0.0522、0.2466±0.0342,CXCR4급CCR7단백표체추세동기상대응적기인표체추세기본일치.4주위암세포침습실험측정적침습세포수분별위:400.0±18.2、310.0±4.0、110.0±13.9、85.0±9.5.CXCR4재불동침습능력적위암세포주중존재차이성표체(P<0.05).결론 CXCR4적표체여위암세포침습능력성정상관,CCR7적표체여위암세포침습능력무명현상관.
Objective To compare the differential expression of chemokine receptors CXCR4 and CCR7 in human gastric carcinoma cell lines with different invasion ability. Methods The expression of CXCR4 and CCR7 in MGC803, AGS, BGC823 and SGC7901 cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The invasion ability of gastric cancer cell lines was measured using transwell chamber with matrigle. Results The expression of CXCR4 mRNA in MGC803, AGS, BGC823 and SGC790 cells was 1. 2556 ±0. 1384, 0. 7943 ± 0. 0913, 0. 4749 ± 0. 0744, 0. 2463 ± 0. 0344, that of CCR7 mRNA was 0. 6071 ± 0. 1404, 0. 5355 ± 0. 0750, 0. 2549 ± 0. 0522, 0. 2466 ± 0. 0342, respectively. The protein expression of CXCR4 and CCR7 in four human gastric carcino-ma cell lines showed a good agreement with mRNA expression. The penetrating cell number of MGC803, AGS, BGC823 and SGC790 was 400. 0 ± 18. 2, 310. 0 ±4. 0, 110. 0 ±13.9, 85.0 ± 9. 5, respectively. The expression levels of CXCR4 in cancer cells showed a significant positive correlation with the invasive capacity (P <0. 05). Conclusion The expression of CXCR4 is positively correlated with cell invasion phenotype in human gastric cancer cells. There was no differential expression of CCR7 in the four gastric cancer cell lines.
