中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
26期
1837-1840
,共4页
赵丹云%段丽萍%朱元莉%李志强
趙丹雲%段麗萍%硃元莉%李誌彊
조단운%단려평%주원리%리지강
应激%胃食管反流%大鼠%细胞外液
應激%胃食管反流%大鼠%細胞外液
응격%위식관반류%대서%세포외액
Stress%Gagtroesophageal reflux%Rats%Extracelluhr fluid
目的 研究慢性束缚应激(CRS)对大鼠食管上皮屏障的影响,探讨精神心理因素在胃食管反流病发病中的作用.方法 成年雄性SD大鼠随机分为4组(各6只):对照组;CRS组(连续束缚应激7 d,每天2 h);酸灌注组(以4 ml/h的速度于麻醉状态下远端食管灌注酸化胃蛋白酶60min);CRS+酸灌注组.光学显微镜观察食管黏膜组织学改变,透射电镜评估食管上皮细胞间隙变化.结果 CRS组大鼠平均每日进食量和7 d体重增长量均显著低于对照组(均P<0.01);CRS组大鼠食管上皮细胞间隙宽于对照组[(0.457±0.031)tun vs(0.235±0.027)μm,P<0.01];对照组和CRS组大鼠酸灌注60 min后,CRS+酸灌注组食管黏膜组织学评分显著高于酸灌注组(2.33±0.33vs 1.33±0.33,P<0.05),两组细胞间隙均增宽,且CRS+酸灌注组较酸灌注组增宽更为明显[(0.516±0.010)μm vs(0.441±0.021)μm,P<0.05].结论 CRS可导致大鼠食管上皮细胞间隙明显增宽,破坏食管上皮屏障功能,产生类似于食管内酸灌注的食管形态学变化,在此基础上的酸灌注可进一步加重黏膜的损伤.为阐明精神心理因素可导致胃食管反流病及功能性烧心患者症状产生和加重的机制提供实验基础.
目的 研究慢性束縳應激(CRS)對大鼠食管上皮屏障的影響,探討精神心理因素在胃食管反流病髮病中的作用.方法 成年雄性SD大鼠隨機分為4組(各6隻):對照組;CRS組(連續束縳應激7 d,每天2 h);痠灌註組(以4 ml/h的速度于痳醉狀態下遠耑食管灌註痠化胃蛋白酶60min);CRS+痠灌註組.光學顯微鏡觀察食管黏膜組織學改變,透射電鏡評估食管上皮細胞間隙變化.結果 CRS組大鼠平均每日進食量和7 d體重增長量均顯著低于對照組(均P<0.01);CRS組大鼠食管上皮細胞間隙寬于對照組[(0.457±0.031)tun vs(0.235±0.027)μm,P<0.01];對照組和CRS組大鼠痠灌註60 min後,CRS+痠灌註組食管黏膜組織學評分顯著高于痠灌註組(2.33±0.33vs 1.33±0.33,P<0.05),兩組細胞間隙均增寬,且CRS+痠灌註組較痠灌註組增寬更為明顯[(0.516±0.010)μm vs(0.441±0.021)μm,P<0.05].結論 CRS可導緻大鼠食管上皮細胞間隙明顯增寬,破壞食管上皮屏障功能,產生類似于食管內痠灌註的食管形態學變化,在此基礎上的痠灌註可進一步加重黏膜的損傷.為闡明精神心理因素可導緻胃食管反流病及功能性燒心患者癥狀產生和加重的機製提供實驗基礎.
목적 연구만성속박응격(CRS)대대서식관상피병장적영향,탐토정신심리인소재위식관반류병발병중적작용.방법 성년웅성SD대서수궤분위4조(각6지):대조조;CRS조(련속속박응격7 d,매천2 h);산관주조(이4 ml/h적속도우마취상태하원단식관관주산화위단백매60min);CRS+산관주조.광학현미경관찰식관점막조직학개변,투사전경평고식관상피세포간극변화.결과 CRS조대서평균매일진식량화7 d체중증장량균현저저우대조조(균P<0.01);CRS조대서식관상피세포간극관우대조조[(0.457±0.031)tun vs(0.235±0.027)μm,P<0.01];대조조화CRS조대서산관주60 min후,CRS+산관주조식관점막조직학평분현저고우산관주조(2.33±0.33vs 1.33±0.33,P<0.05),량조세포간극균증관,차CRS+산관주조교산관주조증관경위명현[(0.516±0.010)μm vs(0.441±0.021)μm,P<0.05].결론 CRS가도치대서식관상피세포간극명현증관,파배식관상피병장공능,산생유사우식관내산관주적식관형태학변화,재차기출상적산관주가진일보가중점막적손상.위천명정신심리인소가도치위식관반류병급공능성소심환자증상산생화가중적궤제제공실험기출.
Objective To investigate the effect of chronic restraint stress(CRS)on the esophageal epithelial barrier function and discuss the role of emotional factors in the pathogenesis of gastroesophageal reflux.Methods Twenty-four male SD rats were randomly divided into 4 equal groups:CRS group submitted to partial restraint stress 2 h per day for 7 days,CBS+acid/pepsin perfusion group submitted to partial restraint stress 2 h per day for 7 days and then anesthetization and perfusion of hydrecldofic acid (pH=2)and pepsin(1 g/L)into the distal esophagus at the speed of 4 ml/h for 60 min,acid/pepsin perfusion group submitted to acid/pepsin perfusion into the esophagus,and control group.Then the rats were killed to collect the specimens of distal esophagus to undergo HE staining and light microscopy.Histological scoring was conducted.The diameter of intercellular spaces of esophageal epithelium wag assessed.Results The amounts offood intake and body weight increment of the CRS and CRS+acid/pepsin perfusion groups were significantly lower than those of the control and acid/pepsin perfusion groups(all P<0.01).The histological score of the CRS+acid/pepsin perfusion group was the highest.The diameter of intercellular spaces of esophagus of the CRS+acid/pepsin perfusion group was(0.516±0.010)μm,significantly greater than those of the CRS,acid/pepsin perfusion,and control groups[(O.457±0.031)μm,(0.441±0.021)μm,and(0.235±0.027)μm respectively,P<0.01 or P<0.05].The histological score of the CRS+acid/pepsin perfusion group was 2.33±0.33,significantly higher than those of the CRS,acid/pepsin peffusion,and control groups(1.17±O.31,1.33±0.33;and 0.33±O.21 respectively,P<0.05 or P<0.01).Conclusion CRS enlarges significantly the esophageal DIS and impairs the epithehal barrier function of the esophagus.Psychological factors induce or enhalice the reflux symptom in the GERD patients.agpeeiaUv in non-erosive reflux disease and functionai heartburn pafients.
