CAJ | 학술논문

比较遗传学研究表明,禾本科不同基因组之间存在着广泛的同线性和共线性.对水稻(Oryza sativa L.)这一模式植物与其他禾本科植物的原位杂交定位可以揭示禾本科植物基因组的共同特点和进化规律,为建立禾本科遗传大体系积累资料.实验以图位克隆法分离的水稻Pib 基因(10.3 kb)和与之连锁的RFLP标记为探针, 研究了Pib及与其连锁的RFLP标记在供试种中的同源性和物理位置. Southern杂交结果表明,Pib在玉米(Zea mays L.)基因组中有同源序列.进一步利用单色和双色荧光原位杂交技术确定了Pib在栽培稻(O.sativa ssp. indica cv. Guangluai 4)、玉米和药用野生稻(O. officinalis Wall ex Watt)染色体上的物理位置.定位结果表明,Pib基因和与之连锁的RFLP标记在这3个供试种基因组中具有同线性.
비교유전학연구표명,화본과불동기인조지간존재착엄범적동선성화공선성.대수도(Oryza sativa L.)저일모식식물여기타화본과식물적원위잡교정위가이게시화본과식물기인조적공동특점화진화규률,위건립화본과유전대체계적루자료.실험이도위극륭법분리적수도Pib 기인(10.3 kb)화여지련쇄적RFLP표기위탐침, 연구료Pib급여기련쇄적RFLP표기재공시충중적동원성화물리위치. Southern잡교결과표명,Pib재옥미(Zea mays L.)기인조중유동원서렬.진일보이용단색화쌍색형광원위잡교기술학정료Pib재재배도(O.sativa ssp. indica cv. Guangluai 4)、옥미화약용야생도(O. officinalis Wall ex Watt)염색체상적물리위치.정위결과표명,Pib기인화여지련쇄적RFLP표기재저3개공시충기인조중구유동선성.
Comparative genetic studies have shown that there are widespread synteny and colinearity of the genes among different species within grass family. Rice (Oryza sativa L.) is a model plant, and analysis of its genome allows us to reveal the common features and the evolutionary rules of the gramineous genomes and accumulate the data for establishment of a common genetic system in the Poaceae. In this study, a rice gene Pib (10.3 kb), a map-based cloned gene, and RFLP markers linked with it are used as the tested probes to investigate their homology and physical location among the tested species. Southern blotting analysis showed that there were sequences homologous to Pib in maize genome. Further, Pib was localized onto the chromosomes of O. Sativa ssp. indica cv. Guangluai 4, O. Officinalis Wall ex Watt and the inbred line of Zea mays cv. Huangzao 4. The results of fluorescence in situ hybridization (FISH) and double-color FISH indicated that a synteny of Pib and RFLP markers linked with Pib existed among the genomes of the three tested species.