中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2003年
13期
1898-1899
,共2页
味觉%原癌基因蛋白质 c-fos类%孤束核
味覺%原癌基因蛋白質 c-fos類%孤束覈
미각%원암기인단백질 c-fos류%고속핵
taste%proto-oncogene proteins c-fos%solitary nucleus
目的观察内脏不适刺激和甜味觉刺激单独给予和先后联合给予对脑干孤束核 (nucleus tractus solitarius,NTS) 中相关神经元的激活作用,为内脏感觉和味觉在 NTS中的信息处理提供形态学方面的资料.方法 SD雄性大鼠 16只,以抽签法随机分为 4组,分别给予 4种不同方式的刺激,即处理组 T1:胃内灌注 LiCl+ 口内灌注糖精 (I/G LiCl+ I/O Sac); T2:胃内灌注 LiCl + 口内灌注蒸馏水 (I/G LiCl+ I/O Water); T3:胃内灌注蒸馏水+ 口内灌注糖精 (I/G Water+ I/O Sac);对照组 C4:胃内灌注蒸馏水+ 口内灌注蒸馏水 (I/G Water+ I/O Water).应用免疫组织化学方法观察刺激后大鼠孤束核吻尾方向上不同区域的 FOS蛋白表达情况.结果与对照组 C4相比, T1组大鼠在孤束核吻尾方向上 5个区域的 FOS蛋白表达增加( P1=0.001, P2=0.000, P3=0.000, P4=0.002, P5=0.002); T2组( P1=0.049, P2=0.001, P3=0.021; P4=0.002; P5=0.001)和 T3组( P1=0.005, P2=0.000, P3=0.031, P4=0.002, P5=0.000)在这些区域的 FOS蛋白表达也增加.两者联合刺激对孤束核尾侧最后区周围的内脏亚核( N2)和吻侧的味觉区( N4,N5)的 FOS蛋白表达有交互作用(分别为 F2=11.87, P2< 0.01; F4=6.83, P4< 0.05; F5=12.81, P5< 0.01).结论不适内脏刺激和甜味觉刺激对孤束核相关神经元的激活具有交互抑制作用.
目的觀察內髒不適刺激和甜味覺刺激單獨給予和先後聯閤給予對腦榦孤束覈 (nucleus tractus solitarius,NTS) 中相關神經元的激活作用,為內髒感覺和味覺在 NTS中的信息處理提供形態學方麵的資料.方法 SD雄性大鼠 16隻,以抽籤法隨機分為 4組,分彆給予 4種不同方式的刺激,即處理組 T1:胃內灌註 LiCl+ 口內灌註糖精 (I/G LiCl+ I/O Sac); T2:胃內灌註 LiCl + 口內灌註蒸餾水 (I/G LiCl+ I/O Water); T3:胃內灌註蒸餾水+ 口內灌註糖精 (I/G Water+ I/O Sac);對照組 C4:胃內灌註蒸餾水+ 口內灌註蒸餾水 (I/G Water+ I/O Water).應用免疫組織化學方法觀察刺激後大鼠孤束覈吻尾方嚮上不同區域的 FOS蛋白錶達情況.結果與對照組 C4相比, T1組大鼠在孤束覈吻尾方嚮上 5箇區域的 FOS蛋白錶達增加( P1=0.001, P2=0.000, P3=0.000, P4=0.002, P5=0.002); T2組( P1=0.049, P2=0.001, P3=0.021; P4=0.002; P5=0.001)和 T3組( P1=0.005, P2=0.000, P3=0.031, P4=0.002, P5=0.000)在這些區域的 FOS蛋白錶達也增加.兩者聯閤刺激對孤束覈尾側最後區週圍的內髒亞覈( N2)和吻側的味覺區( N4,N5)的 FOS蛋白錶達有交互作用(分彆為 F2=11.87, P2< 0.01; F4=6.83, P4< 0.05; F5=12.81, P5< 0.01).結論不適內髒刺激和甜味覺刺激對孤束覈相關神經元的激活具有交互抑製作用.
목적관찰내장불괄자격화첨미각자격단독급여화선후연합급여대뇌간고속핵 (nucleus tractus solitarius,NTS) 중상관신경원적격활작용,위내장감각화미각재 NTS중적신식처리제공형태학방면적자료.방법 SD웅성대서 16지,이추첨법수궤분위 4조,분별급여 4충불동방식적자격,즉처리조 T1:위내관주 LiCl+ 구내관주당정 (I/G LiCl+ I/O Sac); T2:위내관주 LiCl + 구내관주증류수 (I/G LiCl+ I/O Water); T3:위내관주증류수+ 구내관주당정 (I/G Water+ I/O Sac);대조조 C4:위내관주증류수+ 구내관주증류수 (I/G Water+ I/O Water).응용면역조직화학방법관찰자격후대서고속핵문미방향상불동구역적 FOS단백표체정황.결과여대조조 C4상비, T1조대서재고속핵문미방향상 5개구역적 FOS단백표체증가( P1=0.001, P2=0.000, P3=0.000, P4=0.002, P5=0.002); T2조( P1=0.049, P2=0.001, P3=0.021; P4=0.002; P5=0.001)화 T3조( P1=0.005, P2=0.000, P3=0.031, P4=0.002, P5=0.000)재저사구역적 FOS단백표체야증가.량자연합자격대고속핵미측최후구주위적내장아핵( N2)화문측적미각구( N4,N5)적 FOS단백표체유교호작용(분별위 F2=11.87, P2< 0.01; F4=6.83, P4< 0.05; F5=12.81, P5< 0.01).결론불괄내장자격화첨미각자격대고속핵상관신경원적격활구유교호억제작용.
Aim To observe effects of visceral malaise and sweet taste alone and combined respectively in activation of correlated neurons in nucleus tractus solitarius(NTS) and provide morphological data for information processing of visceral and gustatory sensation in NTS.Methods 16 male SD rats were randomly divided into 4 groups by drawing lots and different stimulations were given.Treatment group 1(T1):intragastric infusion of LiCl+ intraoral infusion of saccharin(I/G LiCl+ I/O Sac); T2: intragastric infusion of LiCl + intraoral infusion of distilled water(I/G LiCl+ I/O Water); T3: intragastric infusion of distilled water+ intraoral infusion of saccharin(I/G Water+ I/O Sac); Control group(C4):intragastrically infusion of distilled water+ intraorally infusion of distilled water(I/G Water+ I/O Water).Expression of FOS protein at different regions of rostro-caudal extend in rat NTS was observed by immunohistochemical staining technique.Results Compared with T4,expression of FOS protein at five regions of rostro-caudal extend in rat NTS as increased in T1( P1=0.001, P2=0.000, P3=0.000, P4=0.002, P5=0.002) ;T2( P1=0.049, P2=0.001, P3=0.021; P4=0.002; P5=0.001) and T3( P1=0.005, P2=0.000, P3=0.031, P4=0.002, P5=0.000) .Combined stimulation showed an interaction on expression of FOS protein in peripheral caudal visceral region(N2)and rostral taste region(N4,N5) in NTS(F2=11.87, P2< 0.01; F4=6.83, P4< 0.05; F5=12.81, P5< 0.01).Conclusion Visceral malaise and sweet taste play an interaction in activation of rat NTS.
