CAJ | 학술논문

设计了一种新的诱导型Cre/lox系统,并在转基因烟草(Nicotianatabacum L.)中进行了验证.在诱导剂的作用下,位于同向lox位点之间的选择标记基因(hpt)和重组酶基因(Cre)在烟草愈伤组织中被删除.在该系统中,Cre基因在玉米乙酰苯胺类化合物诱导启动子(In5-2)的控制下表达.对转基因后代的分子检测结果表明,不论是否加入了诱导剂,目的基因(gus)均被整合到烟草基因组中;在诱导剂处理的48株转基因烟草To代中,45株的hpt基因被删除了.该系统只使用一个载体,克服了二次转化系统带来的问题.
설계료일충신적유도형Cre/lox계통,병재전기인연초(Nicotianatabacum L.)중진행료험증.재유도제적작용하,위우동향lox위점지간적선택표기기인(hpt)화중조매기인(Cre)재연초유상조직중피산제.재해계통중,Cre기인재옥미을선분알류화합물유도계동자(In5-2)적공제하표체.대전기인후대적분자검측결과표명,불론시부가입료유도제,목적기인(gus)균피정합도연초기인조중;재유도제처리적48주전기인연초To대중,45주적hpt기인피산제료.해계통지사용일개재체,극복료이차전화계통대래적문제.
A new inducible Cre/lox system was constructed in transgenic tobacco (Nicotiana tabacum L.) plants. The inducer-treatment of tobacco callus mediates an excision event in which the selectable marker gene and Cre gene between two lox sites were deleted. A chloroacetanilide-induced promoter (In5-2) was used to control the expression of Cre gene in this system. Molecular analysis of transgenic tobacco plants showed the interested gene, β-glucuronidase (gus), was integrated into the genome whether removing has been successful, and forty-five out of forty-eight To plants were transgenic tobacco without the marker gene, hpt. This system uses a single vector to circumvent the flaw of other dual recombinase vector systems.