中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2009年
4期
417-419
,共3页
宫颈肿瘤%Hela细胞%野生型p53基因%多西他赛
宮頸腫瘤%Hela細胞%野生型p53基因%多西他賽
궁경종류%Hela세포%야생형p53기인%다서타새
cervical neoplasm%Hela cell%wild type p53 gene (wt p53)%docetaxel
目的 探讨多西他赛单用及与野生型p53基因联合应用对宫颈癌Hela细胞的抑制作用.方法 Hela细胞分两组培养,p53转染实验组(p53-Hela组)和空白对照组(Hela组),野生型p53基因表达用逆转录-多聚酶链反应鉴定.多西他赛作用于p53-Hela细胞及Hela细胞,24小时与48小时后形态学观察并使用活细胞计数试剂盒检测吸光度值OD450,计算细胞抑制率.结果 用逆转录-多聚酶链反应法检测到p53-Hela细胞的p53 mRNA表达,与对照组Hela细胞的p53 mRNA表达相比,差异有统计学意义(t=-17.504,P<0.01).多西他赛对p53-Hela及Hela细胞作用时,不同时间都有抑制作用(Fp53-Hela=53.500,P<0.01;FHela=430.225,P<0.01),该抑制作用呈剂量依赖性和时间依赖性.结论 多西他赛单独应用时可对宫颈癌Hela细胞产生抑制作用,与野生型p53基因联用时,其药物抑制作用增强.
目的 探討多西他賽單用及與野生型p53基因聯閤應用對宮頸癌Hela細胞的抑製作用.方法 Hela細胞分兩組培養,p53轉染實驗組(p53-Hela組)和空白對照組(Hela組),野生型p53基因錶達用逆轉錄-多聚酶鏈反應鑒定.多西他賽作用于p53-Hela細胞及Hela細胞,24小時與48小時後形態學觀察併使用活細胞計數試劑盒檢測吸光度值OD450,計算細胞抑製率.結果 用逆轉錄-多聚酶鏈反應法檢測到p53-Hela細胞的p53 mRNA錶達,與對照組Hela細胞的p53 mRNA錶達相比,差異有統計學意義(t=-17.504,P<0.01).多西他賽對p53-Hela及Hela細胞作用時,不同時間都有抑製作用(Fp53-Hela=53.500,P<0.01;FHela=430.225,P<0.01),該抑製作用呈劑量依賴性和時間依賴性.結論 多西他賽單獨應用時可對宮頸癌Hela細胞產生抑製作用,與野生型p53基因聯用時,其藥物抑製作用增彊.
목적 탐토다서타새단용급여야생형p53기인연합응용대궁경암Hela세포적억제작용.방법 Hela세포분량조배양,p53전염실험조(p53-Hela조)화공백대조조(Hela조),야생형p53기인표체용역전록-다취매련반응감정.다서타새작용우p53-Hela세포급Hela세포,24소시여48소시후형태학관찰병사용활세포계수시제합검측흡광도치OD450,계산세포억제솔.결과 용역전록-다취매련반응법검측도p53-Hela세포적p53 mRNA표체,여대조조Hela세포적p53 mRNA표체상비,차이유통계학의의(t=-17.504,P<0.01).다서타새대p53-Hela급Hela세포작용시,불동시간도유억제작용(Fp53-Hela=53.500,P<0.01;FHela=430.225,P<0.01),해억제작용정제량의뢰성화시간의뢰성.결론 다서타새단독응용시가대궁경암Hela세포산생억제작용,여야생형p53기인련용시,기약물억제작용증강.
Objective To investigate inhibitory effects of docetaxel alone and docetaxel combined with wild type p53(wt p53) on proliferation of human cervical cancer cells-Hela cells. Methods The Hela cells were divided into two groups: wt p53 transfected Hela cells group(p53-Hela group, obtained by using lipofection-mediated gene transfection method) and blank control group (not transfected with wt p53). The expression of wt p53 was deteced by using reverse transcription polymerase chain reaction(RT-PCR) method. Docetaxel acted on Hela cells and p53-Hela cells. After 24 hours and 48 hours,the morphology of the cells in the two groups were observed respectively and OD450 were tested by using cell counting kit-8(CCK-8) to caculate the inhibition rate. Results After transfection with wt p53, the expression of p53mRNA in p53-Hela group was increased, and there was a significant difference as compared with that in Hela group (t=-17.504, P<0.01). Docetaxel exerted an inhibitary effect on the Hela cell line in the two groups at all action times (Fp53-Hela=53.500,P<0.01;FHela=430.225,P<0.01). This cellular inhibitory effects showed dose and time dependent manners. Conclusion Docetaxel alone exerts an inhibitory effect on Hela cells and this inhibitary effect could be increased when docetaxel is combined with wt p53.
