CAJ | 학술논문

对丁香抗氧化活性物质提取工艺及其抗氧化活性进行了研究.结果表明,最佳提取工艺条件为:60%乙醇、提取温度60℃、料液比1∶20和提取时间40 min;在此工艺条件下抗氧化活性物质提取率为(10 480.4±40.3)μmol/g,获得的相应丁香粗提物具有较强的抗氧化活性,总还原力弱于相同质量浓度的阳性对照BHT和VC(P<0.01或P<0.001),FRAP法抗氧化能力和DPPH自由基清除能力弱于相同质量浓度的VC,强于相同质量浓度的BHT(P<0.05、P<0.01或P<0.001);通过生物活性追踪发现丁香抗氧化活性物质主要存在于其弱极性的乙酸乙酯部分,该部分100 μg/mL质量浓度样液的总还原力达0.634±0.040,FRAP法抗氧化能力达0.433±0.005,DPPH自由基清除率达(85.294±0.499)%;相关关系表明丁香抗氧化活性的主要物质基础为总多酚和总黄酮.
대정향항양화활성물질제취공예급기항양화활성진행료연구.결과표명,최가제취공예조건위:60%을순、제취온도60℃、료액비1∶20화제취시간40 min;재차공예조건하항양화활성물질제취솔위(10 480.4±40.3)μmol/g,획득적상응정향조제물구유교강적항양화활성,총환원력약우상동질량농도적양성대조BHT화VC(P<0.01혹P<0.001),FRAP법항양화능력화DPPH자유기청제능력약우상동질량농도적VC,강우상동질량농도적BHT(P<0.05、P<0.01혹P<0.001);통과생물활성추종발현정향항양화활성물질주요존재우기약겁성적을산을지부분,해부분100 μg/mL질량농도양액적총환원력체0.634±0.040,FRAP법항양화능력체0.433±0.005,DPPH자유기청제솔체(85.294±0.499)%;상관관계표명정향항양화활성적주요물질기출위총다분화총황동.
The extracting technology and antioxidant activity of bioactive components from clove were studied. The results showed that the optimal parameters of the extracting technology were as follows, the concentration of ethanol was 60%,the extracting temperature was 60℃,the ratio of sample to extracting solution was 1∶20 , and the extracting time was 40 min. The recovery ratio of bioactive compounds from clove was (10 480.4±40.3) μmol/g in this extracting technology. The crude extracts obtained from this extracting technology were proved to have strong antioxidant activity. The reducibility of the crude extracts was weaker than those of two positive controls (BHT and VC) (P<0.01 or P<0.001). The FRAP values and the activity of scavenging DPPH radicals of the crude extracts were weaker than those of VC and stronger than those of BHT (P<0.05, P<0.01 or P<0.001). It was found by using the bioassay guided method that the bioactive antioxidants of clove were mainly attributed to the ethyl acetate fraction. The reducibility (OD 700 nm) and FRAP values (OD 593 nm) of the sample of the ethyl acetate fraction with the concentration of 100 μg/mL were 0.634±0.040 and 0.433±0.005, respectively. The percentage of scavenging DPPH radicals of the same sample was (85.294±0.499)%. The result of correlation analysis showed that the strong antioxidant activity mainly resulted from the high contents of the total phenols and total flavonoids.