CAJ | 학술논문

建立一种基于反相高效液相色谱(RP-HPLC)的分析方法,对牛奶及其乳制品进行定量分析.此方法可一次分离牛奶及乳制品中的7种主要蛋白成分(κ-CN、α_s2-CN、α_s1-CN、β-CN、α-La、β-LgB、β-LgA),解决了加工后乳制品中变性蛋白定量不准确的问题和κ-CN、α_s-CN以及乳清蛋白中α-La、β-LgB和β-LgA难以分离的困难.各组分分离度均大于1,达到基线分离,回收率达88.9%～97.1%,相对标准偏差为0.8%～5.1%.该方法小需对样品进行离心,在测定清蛋白组分时,也不需提前去除优势蛋白酪蛋白,与传统的检测方法相比,具有准确、灵敏和快速等特点,可用于牛奶及其乳制品的质最控制和定量检测.
건립일충기우반상고효액상색보(RP-HPLC)적분석방법,대우내급기유제품진행정량분석.차방법가일차분리우내급유제품중적7충주요단백성분(κ-CN、α_s2-CN、α_s1-CN、β-CN、α-La、β-LgB、β-LgA),해결료가공후유제품중변성단백정량불준학적문제화κ-CN、α_s-CN이급유청단백중α-La、β-LgB화β-LgA난이분리적곤난.각조분분리도균대우1,체도기선분리,회수솔체88.9%～97.1%,상대표준편차위0.8%～5.1%.해방법소수대양품진행리심,재측정청단백조분시,야불수제전거제우세단백락단백,여전통적검측방법상비,구유준학、령민화쾌속등특점,가용우우내급기유제품적질최공제화정량검측.
Totally seven main proteins (κ-CN, α_s-CN, α_s1-CN, β-CN, α-La, β-LgB, β-LgA) in milk and dairy products were simultaneously determined with reverse-phase HPLC method. This method successfully solved the most concerned problems in traditional methods such as inaccurate quantitative analysis of degradative protein in processed dairy products and the difficulty of separating κ-CN and α_s2-CN, as well as α-La,β-LgB and β-LgA in whey protein. The chromatographic resolution of each component is more than 1. and baseline separation was achieved. The recovery rate was about 88.9%-97.1% and the value of relative standard deviation (RSD) was 0.8%-5.1%. In addition, the sample centrifuging process and the casein removal step in albumin analysis were not needed any more. Compared with previous methods, the method was sensitive, accurate and rapid and could be used for quality control and quantitative analysis of milk or dairy products.