CAJ | 학술논문

建立了鳗鲡肌肉冻干粉中3-氨基-2-(噁)唑烷酮(AOZ)残留分析标准物质的研制和定值方法.以约0.16 mg/L呋喃唑酮对鳗鲡进行药浴给药,使呋喃唑酮在鱼体中代谢,从而使鳗鲡肌肉中含有一定浓度的呋喃唑酮代谢物AOZ.经冻干、真空包装及辐照等处理后获得1批400个独立包装的肌肉冻干粉样本.经过均匀性与稳定性检验,由11家实验室采用同位素稀释液相色谱-串联质谱法进行协同定值,并进行了不确定度评估,已被国家质检总局批准确为国家二级标准物质.
건립료만려기육동간분중3-안기-2-(오)서완동(AOZ)잔류분석표준물질적연제화정치방법.이약0.16 mg/L부남서동대만려진행약욕급약,사부남서동재어체중대사,종이사만려기육중함유일정농도적부남서동대사물AOZ.경동간、진공포장급복조등처리후획득1비400개독립포장적기육동간분양본.경과균균성여은정성검험,유11가실험실채용동위소희석액상색보-천련질보법진행협동정치,병진행료불학정도평고,이피국가질검총국비준학위국가이급표준물질.
A methodology for preparing and certifying the reference material of 3-amino-2-oxazolidinone(AOZ) in eel muscle lyophilisates was presented. Furazolidone was accessed to eel by dipping fish in pond with furazolidone solution at a dosage of ca 0.16 mg/L. With the metabolism of furazolidone in eel, the muscles contain a certain concentration of AOZ as furazolidone metabolite was obtained. Lyophilization of the muscles was performed in one batch and 400 bags of samples were obtained by the procedure of homogenation, cryodesiccation and irradiation. The homogeneity and stability of the sample was examined. The value of the chemical constituent of the sample was certified through the collaborative analysis program participated by 11 laboratories using isotope dilution liquid chromatography-tandem mass spectrometry, and the uncertainty assessment was performed. The reference materials have been approved as certified reference materials by AQSIQ, China (State General Administration of the People′s Republic of China for Quality Supervision and Inspection and Quarantine) in 2009 after one year of trial period. The serial numbers is GBW(E)100180.