环境污染与防治
環境汙染與防治
배경오염여방치
ENVIRONMENTAL POLLUTION AND CONTROL
2010年
2期
46-50
,共5页
人工湿地%聚合酶链反应-变性梯度凝胶电泳%微生物群落
人工濕地%聚閤酶鏈反應-變性梯度凝膠電泳%微生物群落
인공습지%취합매련반응-변성제도응효전영%미생물군락
constructed wetland%PCR-DGGE%microorganism community
采用聚合酶链反应(PCR)-变性梯度凝胶电泳(DGGE)技术对梯田式人工湿地(TTCW)和复合垂直流式人工湿地(IVCW)生活污水处理系统内的微生物群落结构的动态变化及其对生活污水处理效果的影响进行了对比研究.结果表明:(1)TTCW和IVCW对生活污水均具有较好处理效果,其COD去除率平均分别为76.2 %和72.6% 、TP去除率平均分别为90.7 %和85.6 %、NH_4~+-N去除率平均分别为65.2 %和58.8%;TTCW增氧作用明显.(2)2种人工湿地内均存在丰富的微生物群落,并均存在共有的微生物种群;在湿地沿程不同位置均存在一定数量的优势种群.(3)TTCW强化了大气复氧能力,使得湿地内DO较相同深度的IVCW的高.TTCW具有较强的污染物去除能力,特别是具有高效的脱氮除磷能力.这种能力可能主要是TTCW中微生物群落结构的变化所致.
採用聚閤酶鏈反應(PCR)-變性梯度凝膠電泳(DGGE)技術對梯田式人工濕地(TTCW)和複閤垂直流式人工濕地(IVCW)生活汙水處理繫統內的微生物群落結構的動態變化及其對生活汙水處理效果的影響進行瞭對比研究.結果錶明:(1)TTCW和IVCW對生活汙水均具有較好處理效果,其COD去除率平均分彆為76.2 %和72.6% 、TP去除率平均分彆為90.7 %和85.6 %、NH_4~+-N去除率平均分彆為65.2 %和58.8%;TTCW增氧作用明顯.(2)2種人工濕地內均存在豐富的微生物群落,併均存在共有的微生物種群;在濕地沿程不同位置均存在一定數量的優勢種群.(3)TTCW彊化瞭大氣複氧能力,使得濕地內DO較相同深度的IVCW的高.TTCW具有較彊的汙染物去除能力,特彆是具有高效的脫氮除燐能力.這種能力可能主要是TTCW中微生物群落結構的變化所緻.
채용취합매련반응(PCR)-변성제도응효전영(DGGE)기술대제전식인공습지(TTCW)화복합수직류식인공습지(IVCW)생활오수처리계통내적미생물군락결구적동태변화급기대생활오수처리효과적영향진행료대비연구.결과표명:(1)TTCW화IVCW대생활오수균구유교호처리효과,기COD거제솔평균분별위76.2 %화72.6% 、TP거제솔평균분별위90.7 %화85.6 %、NH_4~+-N거제솔평균분별위65.2 %화58.8%;TTCW증양작용명현.(2)2충인공습지내균존재봉부적미생물군락,병균존재공유적미생물충군;재습지연정불동위치균존재일정수량적우세충군.(3)TTCW강화료대기복양능력,사득습지내DO교상동심도적IVCW적고.TTCW구유교강적오염물거제능력,특별시구유고효적탈담제린능력.저충능력가능주요시TTCW중미생물군락결구적변화소치.
The dynamic changes of microorganism community and its impact on the pollutant removal efficiency in both the terrace-type constructed wetland (TTCW) and integrated vertical-flow constructed wetland (IVCW) trea-ting domestic sewage were investigated by employing polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that both of TTCW and IVCW presented perfect efficiency of domestic sewage treatment, the average COD removal rate were 76.2% and 72.6%, average TP removal rate were 90.7% and 85.6% average NH_4~+-N removal rate were 65.2% and 58.8%, respectively. More oxygen was produced in TTCW. There were various microorganisms were found in TTCW and IVCW, and some kinds of common microorganisms shared in both the wetland systems, the dominant and functional microorganism community changed along the direction of water flow. TTCW enhanced the reoxygen capacity of atmosphere, therefore the concentration of dissolved oxygen in TTCW were higher than that in IVCW of the same depth. TTCW showed strong capability of pollutant removal, especially for nitrogen and phosphor removal, this might caused by the variation of microorganism community in TTCW.