CAJ | 학술논문

目的:探讨辛伐他汀对大鼠心肌梗死后心肌胶原含量的影响及其机制. 方法:建立大鼠心肌梗死模型,24 h后存活大鼠随机分成心肌梗死组(n=9)、辛伐他汀20 mg组[20 ms/(kg·d),n=10]和平伐他汀40 mg组[40 ms/(kg·d),n=9],另设假手术组(n=10).4周后观察血脂水平、左心室重指数和天狼猩红染色分析左心室非梗死区心肌胶原容积分数(表达心肌胶原含量),免疫组化检测基质金属蛋白酶-2(MMP-2),免疫印迹杂交方法(Western blot)和逆转录多聚酶链反应(RT-PCR)检测转化生长因子β1(TGF-β1)在非梗死区的表达. 结果:①各组血脂水平差异无统计学意义,心肌梗死组左心室重苗指数、非梗死区Ⅰ型、Ⅲ型胶原容积分数及Ⅰ型与Ⅲ型胶原容积分数比值较假手术组均明显升高(P均<0.05),差异均有统计学意义;辛伐他汀两组左心室重量指数、非梗死区Ⅰ型、Ⅲ型胶原容积分数及Ⅰ型与Ⅲ型胶原容积分数比值较心肌梗死组均卜降,但仍高于假手术组(P均<0.05),差异均有统计学意义.②心肌梗死组MMP-2和TGF-β1表达较假手术组均显著增加(P均<0.05),而辛伐他汀两组表达则明显降低,但仍高于假手术组(P均<0.05),差异均有统计学意义. 结论:辛伐他汀能有效改善大鼠心肌梗死后心肌胶原含量,机制与其渊脂作用无关,可能与下调MMP-2和TGF-β1的表达有关.
목적:탐토신벌타정대대서심기경사후심기효원함량적영향급기궤제. 방법:건립대서심기경사모형,24 h후존활대서수궤분성심기경사조(n=9)、신벌타정20 mg조[20 ms/(kg·d),n=10]화평벌타정40 mg조[40 ms/(kg·d),n=9],령설가수술조(n=10).4주후관찰혈지수평、좌심실중지수화천랑성홍염색분석좌심실비경사구심기효원용적분수(표체심기효원함량),면역조화검측기질금속단백매-2(MMP-2),면역인적잡교방법(Western blot)화역전록다취매련반응(RT-PCR)검측전화생장인자β1(TGF-β1)재비경사구적표체. 결과:①각조혈지수평차이무통계학의의,심기경사조좌심실중묘지수、비경사구Ⅰ형、Ⅲ형효원용적분수급Ⅰ형여Ⅲ형효원용적분수비치교가수술조균명현승고(P균<0.05),차이균유통계학의의;신벌타정량조좌심실중량지수、비경사구Ⅰ형、Ⅲ형효원용적분수급Ⅰ형여Ⅲ형효원용적분수비치교심기경사조균복강,단잉고우가수술조(P균<0.05),차이균유통계학의의.②심기경사조MMP-2화TGF-β1표체교가수술조균현저증가(P균<0.05),이신벌타정량조표체칙명현강저,단잉고우가수술조(P균<0.05),차이균유통계학의의. 결론:신벌타정능유효개선대서심기경사후심기효원함량,궤제여기연지작용무관,가능여하조MMP-2화TGF-β1적표체유관.
Objective:To investigate the beneficial effects of simvastatin on myocardial collagen changes in rats after myocardial infarction (MI) with its possible mechanisms.Methods:MI model was created by the ligation of left anterior descending coronary artery. 24 hours after MI,the survival rats were randomly divided into three groups, MI group(n = 9),Simvastatin 20 mg/( kg·d) treatment group(n = 10) , Simvastatin 40 mg/ ( kg ·d) treatment group (n = 9);meanwhile, the Sham-operation group (n = 10) was established. After 4 weeks treatment , the effects of simvatatin on ventricular remodeling were evaluated by detecting the changes of left ventricular weight index (LVWI) ,the collagen volume fraction (CVF) in non-infarction zone (NIZ)was examined with Picric-Sirius Red Polarimetry,the expression of matrix metalloproteinase-2 ( MMP-2) by immunohistochemistry,and the expression of transforming growth factor β1 (TGF-β1) in N1Z by RT-PCR and Western blot analysis.Meanwhile, the serum lipid levels were examined in all groups.Results:There were no significant changes among different groups for serum lipid levels(P >0. 05). Compared with Sham operation group,LVWI,type Ⅰ CVF,type Ⅲ CVF and the Ⅰ /Ⅲ ratio in NIZ were increased significantly in MI group. Compared with MI group, LVWI, type Ⅰ CVF, type Ⅲ CVF and Ⅰ /Ⅲ ratio in NIZ were decreased significantly in both of Simvastatin treatment groups,but higher than those in Sham operation group. In contrast with MI group,the expressions of MMP-2 and TGF-pi were down regulated in both Simvastatin treatment groups, but higher than those in Sham operation group.Conclusion:Simvastatin could ameliorate MI induced myocardial collagen changes in rats,and this was independent of lipid regulation while it could be associated with down regulated MMP-2 and TGF-β1.