板口线虫,美洲%感染%变态反应和免疫学%皮肤%病理学
闆口線蟲,美洲%感染%變態反應和免疫學%皮膚%病理學
판구선충,미주%감염%변태반응화면역학%피부%병이학
Necator americanus%Infection%Allergry and immunity%Skin%Pathology
目的 观察仓鼠感染美洲钩虫第3期感染性幼虫(NaL3)后攻击感染NaL3时其皮肤组织病理改变以及NaL3的组织形态学变化,寻找仓鼠感染NaL3后对攻击感染NaL3的获得性免疫证据.方法 将仓鼠分为攻击感染组和对照组.攻击感染组仓鼠先经皮肤感染NaL3 150条,2周后与对照组同时感染NaL3 900条并于感染后6,24,72 h和1,2同时取感染部位皮肤,观察组织病理反应以及NaL3的组织形态变化.结果 对照组6,24,72 h和1,2周的NaL3的切面数分别为15,33,11,0,0个,其变性和死亡虫体切面数均为0个.所有虫体切面形态结构完整清晰,皮肤组织亦未出现明显的炎细胞反应.攻击感染组6,24,72 h和1,2周的NaL3的切面数分别为25,53,15,5,4个,变性虫体切面数为0,24,6,0,0个,死亡虫体切面数为0,0,7,5,4个.24 h起虫体角皮粗糙,肿胀,72 h虫体内部结构疏松,细胞核固缩,甚至模糊不清,1周后虫体切面遭明显破坏,隐约可见其残骸.皮肤组织内炎症反应主要有巨噬细胞,类上皮细胞和嗜酸性粒细胞浸润以及死虫肉芽肿形成.结论 仓鼠感染NaL3后埘攻击感染NaL3产生了抵抗力,其获得性免疫反应程度强烈.
目的 觀察倉鼠感染美洲鉤蟲第3期感染性幼蟲(NaL3)後攻擊感染NaL3時其皮膚組織病理改變以及NaL3的組織形態學變化,尋找倉鼠感染NaL3後對攻擊感染NaL3的穫得性免疫證據.方法 將倉鼠分為攻擊感染組和對照組.攻擊感染組倉鼠先經皮膚感染NaL3 150條,2週後與對照組同時感染NaL3 900條併于感染後6,24,72 h和1,2同時取感染部位皮膚,觀察組織病理反應以及NaL3的組織形態變化.結果 對照組6,24,72 h和1,2週的NaL3的切麵數分彆為15,33,11,0,0箇,其變性和死亡蟲體切麵數均為0箇.所有蟲體切麵形態結構完整清晰,皮膚組織亦未齣現明顯的炎細胞反應.攻擊感染組6,24,72 h和1,2週的NaL3的切麵數分彆為25,53,15,5,4箇,變性蟲體切麵數為0,24,6,0,0箇,死亡蟲體切麵數為0,0,7,5,4箇.24 h起蟲體角皮粗糙,腫脹,72 h蟲體內部結構疏鬆,細胞覈固縮,甚至模糊不清,1週後蟲體切麵遭明顯破壞,隱約可見其殘骸.皮膚組織內炎癥反應主要有巨噬細胞,類上皮細胞和嗜痠性粒細胞浸潤以及死蟲肉芽腫形成.結論 倉鼠感染NaL3後塒攻擊感染NaL3產生瞭牴抗力,其穫得性免疫反應程度彊烈.
목적 관찰창서감염미주구충제3기감염성유충(NaL3)후공격감염NaL3시기피부조직병리개변이급NaL3적조직형태학변화,심조창서감염NaL3후대공격감염NaL3적획득성면역증거.방법 장창서분위공격감염조화대조조.공격감염조창서선경피부감염NaL3 150조,2주후여대조조동시감염NaL3 900조병우감염후6,24,72 h화1,2동시취감염부위피부,관찰조직병리반응이급NaL3적조직형태변화.결과 대조조6,24,72 h화1,2주적NaL3적절면수분별위15,33,11,0,0개,기변성화사망충체절면수균위0개.소유충체절면형태결구완정청석,피부조직역미출현명현적염세포반응.공격감염조6,24,72 h화1,2주적NaL3적절면수분별위25,53,15,5,4개,변성충체절면수위0,24,6,0,0개,사망충체절면수위0,0,7,5,4개.24 h기충체각피조조,종창,72 h충체내부결구소송,세포핵고축,심지모호불청,1주후충체절면조명현파배,은약가견기잔해.피부조직내염증반응주요유거서세포,류상피세포화기산성립세포침윤이급사충육아종형성.결론 창서감염NaL3후시공격감염NaL3산생료저항력,기획득성면역반응정도강렬.
Objective To obtain acquired immunity evidence in hamsters elicited by third stage hookworm larvae of Necator americanas(NaL3).morphology changes of NaL3 and inflammatory responses in the skin and undedying subcutaneous tissue and muscles of hamsters were observed.Methods Hamsters were immunized subcutaneously with one dose of 150 NaL3 at 2 weeks earlier,and then challenged pereutaneously with 900 NaL3.Skins were excised from post-challenge hamsters at 6,24,72 hours and 1,2 weeks,and then examined under light microscopy.Non-immunized hamsters served as negative controls.Results In non-immunized hamsters the number of NaL3 were 15,33,11.0 and 0 at 6,24,72 hours and 1,2 weeks post-infection.No damaged or dead NaL3 section was observed.All NaL3 exhibited no structural damage and infihrating inflammatory cells were absent from the sunDunding tissues.There were no cutaneous changes.In contrast.the total number of Nak sections in the skin of immunized hamsters were 25,53,15,5 and 4 at 6,24,72 hours and 1,2 weeks post-challenge.Among these NaL3 sections,damaged and dead section number were 0,24,6,0,0 and 0,0,7,5,4.At 24 hours post-challenge the Nak exhibited cutieular swelling and damage.By 72 hours post-challenge pyknosis of the somatic cells nuclei and sparseness or loss of definition in the internal structures of NaL3 were seen.One or two weeks after chanenge,the NaL3 showed severe damage or even dead with remnants.Inflammatory responses including macrophages,epithelioid cells and eosinophils infiltrating and granulomata forming were mainly seen around the NaL3 sections in the skin of immunized hamsters.Conclusions Hamsters initially immunized with NaL3 exhibited obvious acquired immunity protection against percutaneously challenged infection as evidenced by vigorous inflammatory responses in the skin and underlying subcutaneous tissue and muscle.
